| Effects of digitalis intoxication on pacemaker rhythm and synchronization in rabbit sinus node. | |
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MedLine Citation:
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PMID: 3963214 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Toxic doses of digitalis can induce severe arrhythmias in the sinus node. Since digitalis is known to induce electrical uncoupling in heart tissues, it is possible that the arrhythmias may be mediated by cell-to-cell uncoupling leading to desynchronization of pacemaker cells. We investigated this possibility using intracellular recordings and two different preparations of isolated rabbit sinus node. First, the whole sinus node region with the crista terminalis and a portion of right atrium were exposed to 2 microM ouabain in the presence of atropine (0.09 microM) and propranolol (0.77 microM). With ouabain superfusion, there was a gradual increase in spontaneous discharge, conduction delay, pacemaker shifts, and arrhythmias associated with multiple pacemaker sites. Interaction between pacemakers rapidly decreased, and finally atrial arrest was observed. Second, thin strips of sinus node were placed in a three-compartment bath. Superfusion of the central segment (1.0-1.5 mm) with 1-5 microM ouabain led to tachycardia and apparent conduction delay, followed by asynchronous firing and phasic interactions between pacemakers across the central segment. These interactions diminished in the course of intoxication, indicating a gradual increase of electrical uncoupling induced by the drug. All effects were reversible on washout. We conclude that digitalis-induced arrhythmias in the sinus node result from cell-to-cell uncoupling and loss of synchrony between pacemaker cells. The pattern of arrhythmias can be predicted by the phasic interactions between the pacemakers. |
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Authors:
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K Takayanagi; J Jalife |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 250 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1986 Apr |
Date Detail:
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Created Date: 1986-05-13 Completed Date: 1986-05-13 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: H567-78 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Action Potentials
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drug effects Animals Arrhythmias, Cardiac / chemically induced Cell Communication / drug effects Female Male Ouabain / pharmacology*, toxicity Rabbits Sinoatrial Node / drug effects* Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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HL-29439/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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630-60-4/Ouabain |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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