Document Detail


Effects of digesting chondroitin sulfate proteoglycans on plasticity in cat primary visual cortex.
MedLine Citation:
PMID:  23283337     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Monocular deprivation (MD) during a critical period of postnatal development produces significant changes in the anatomy and physiology of the visual cortex, and the deprived eye becomes amblyopic. Extracellular matrix molecules have a major role in restricting plasticity such that the ability to recover from MD decreases with age. Chondroitin sulfate proteoglycans (CSPGs) act as barriers to cell migration and axon growth. Previous studies showing that degradation of CSPGs by the bacterial enzyme chondroitinase can restore plasticity in the adult rat visual cortex suggest a potential treatment for amblyopia. Here MD was imposed in cats from the start of the critical period until 3.5 months of age. The deprived eye was reopened, the functional architecture of the visual cortex was assessed by optical imaging of intrinsic signals, and chondroitinase was injected into one hemisphere. Imaging was repeated 1 and 2 weeks postinjection, and visually evoked potentials (VEPs) and single-cell activity were recorded. Immunohistochemistry showed that digestion of CSPGs had been successful. After 2 weeks of binocular exposure, some recovery of deprived-eye responses occurred when chondroitinase had been injected into the hemisphere contralateral to that eye; when injected into the ipsilateral hemisphere, no recovery was seen. Deprived-eye VEPs were no larger in the injected hemisphere than in the opposite hemisphere. The small number of neurons dominated by the deprived eye exhibited poor tuning characteristics. These results suggest that despite structural effects of chondroitinase in adult cat V1, plasticity was not sufficiently restored to enable significant functional recovery of the deprived eye.
Authors:
Vasily Vorobyov; Jessica C F Kwok; James W Fawcett; Frank Sengpiel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  33     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-03     Completed Date:  2013-03-12     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  234-43     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amblyopia / metabolism,  physiopathology
Animals
Brain Mapping
Cats
Chondroitin Sulfate Proteoglycans / metabolism*
Chondroitinases and Chondroitin Lyases / pharmacology*
Evoked Potentials, Visual / physiology
Female
Male
Neuronal Plasticity / drug effects*,  physiology
Sensory Deprivation / physiology*
Visual Cortex / drug effects*,  metabolism,  physiopathology
Grant Support
ID/Acronym/Agency:
G0502299//Medical Research Council; G9724886//Medical Research Council
Chemical
Reg. No./Substance:
0/Chondroitin Sulfate Proteoglycans; EC 4.2.2.-/Chondroitinases and Chondroitin Lyases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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