Document Detail


Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts.
MedLine Citation:
PMID:  20872988     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To investigate the influence of microwave radiation on the human fibroblast nuclei, the effects of three variants of electromagnetic wave polarization, linear and left-handed and right-handed elliptically polarized, were examined. Experimental conditions were: frequency (f) 36.65 GHz, power density (P) at the surface of exposed object 1, 10, 30, and 100 µW/cm(2), exposure time 10 s. Human fibroblasts growing in a monolayer on a cover slide were exposed to microwave electromagnetic radiation. The layer of medium that covered cells during microwave exposure was about 1 mm thick. Cells were stained immediately after irradiation by 2% (w/v) orcein solution in 45% (w/v) acetic acid. Experiments were made at room temperature (25 °C), and control cell samples were processed in the same conditions. We assessed heterochromatin granule quantity (HGQ) at 600× magnification. Microwave irradiation at the intensity of 1 µW/cm(2) produced no effect, and irradiation at the intensities of 10 and 100 µW/cm(2) induced an increase in HGQ. More intense irradiation induced more chromatin condensation. The right-handed elliptically polarized radiation revealed more biological activity than the left-handed polarized one.
Authors:
Yuriy G Shckorbatov; Vladimir N Pasiuga; Elena I Goncharuk; Tatiana Ph Petrenko; Valentin A Grabina; Nicolay N Kolchigin; Dmitry D Ivanchenko; Victor N Bykov; Oleksandr M Dumin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of Zhejiang University. Science. B     Volume:  11     ISSN:  1862-1783     ISO Abbreviation:  J Zhejiang Univ Sci B     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-27     Completed Date:  2011-01-21     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  101236535     Medline TA:  J Zhejiang Univ Sci B     Country:  China    
Other Details:
Languages:  eng     Pagination:  801-5     Citation Subset:  IM    
Affiliation:
Kharkiv National University, pl. Svobody 4, Kharkiv 61077, Ukraine. Yury.G.Shckorbatov@univer.kharkov.ua
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MeSH Terms
Descriptor/Qualifier:
Cell Nucleus / radiation effects*,  ultrastructure
Cells, Cultured
Fibroblasts / radiation effects*,  ultrastructure
Heterochromatin / radiation effects
Humans
Microwaves*
Chemical
Reg. No./Substance:
0/Heterochromatin
Comments/Corrections

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