| Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts. | |
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MedLine Citation:
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PMID: 20872988 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To investigate the influence of microwave radiation on the human fibroblast nuclei, the effects of three variants of electromagnetic wave polarization, linear and left-handed and right-handed elliptically polarized, were examined. Experimental conditions were: frequency (f) 36.65 GHz, power density (P) at the surface of exposed object 1, 10, 30, and 100 µW/cm(2), exposure time 10 s. Human fibroblasts growing in a monolayer on a cover slide were exposed to microwave electromagnetic radiation. The layer of medium that covered cells during microwave exposure was about 1 mm thick. Cells were stained immediately after irradiation by 2% (w/v) orcein solution in 45% (w/v) acetic acid. Experiments were made at room temperature (25 °C), and control cell samples were processed in the same conditions. We assessed heterochromatin granule quantity (HGQ) at 600× magnification. Microwave irradiation at the intensity of 1 µW/cm(2) produced no effect, and irradiation at the intensities of 10 and 100 µW/cm(2) induced an increase in HGQ. More intense irradiation induced more chromatin condensation. The right-handed elliptically polarized radiation revealed more biological activity than the left-handed polarized one. |
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Authors:
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Yuriy G Shckorbatov; Vladimir N Pasiuga; Elena I Goncharuk; Tatiana Ph Petrenko; Valentin A Grabina; Nicolay N Kolchigin; Dmitry D Ivanchenko; Victor N Bykov; Oleksandr M Dumin |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of Zhejiang University. Science. B Volume: 11 ISSN: 1862-1783 ISO Abbreviation: J Zhejiang Univ Sci B Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-27 Completed Date: 2011-01-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101236535 Medline TA: J Zhejiang Univ Sci B Country: China |
Other Details:
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Languages: eng Pagination: 801-5 Citation Subset: IM |
Affiliation:
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Kharkiv National University, pl. Svobody 4, Kharkiv 61077, Ukraine. Yury.G.Shckorbatov@univer.kharkov.ua |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Nucleus
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radiation effects*,
ultrastructure Cells, Cultured Fibroblasts / radiation effects*, ultrastructure Heterochromatin / radiation effects Humans Microwaves* |
| Chemical | |
Reg. No./Substance:
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0/Heterochromatin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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