| Effects of differentiation on the phospholipid and phospholipid fatty acid composition of N1E-115 neuroblastoma cells. | |
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MedLine Citation:
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PMID: 8466939 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of differentiation on the phospholipid and phospholipid fatty acid composition of N1E-115 neuroblastoma cells were determined. The cellular lipids were extracted on days 0, 3 and 7, following the addition of 1.2% dimethylsulfoxide to induce cellular differentiation. Proportions of ethanolamine glycerophospholipids (EtnGpl), phosphatidylinositol (PtdIns) and sphingomyelin (CerPCho) were significantly elevated following differentiation. The mole percentage of choline glycerophospholipids (ChoGpl) decreased with differentiation. The plasmalogens, both choline and ethanolamine, increased by 1.3- and 2.3-fold, respectively, during differentiation. The fatty acid composition of the phospholipid classes was also altered. PtdIns and ChoGpl had decreased proportions of polyenoic fatty acids, while these proportions were increased in EtnGpl. Both ChoGpl and EtnGpl had increased n-3/n-6 series fatty acid ratios, but this ratio was decreased in PtdIns. The mole percentage of arachidonic acid was significantly decreased in both PtdIns and ChoGpl, but elevated in EtnGpl and may be a result of the increase in ethanolamine plasmalogen. Thus, differentiation did not increase the overall mole percentage of polyenoic FA in the cells nor increase the n-6 series fatty acid proportions. We speculate plasmalogens may have a role in the differentiation process or in maintaining the cell in the differentiated state. |
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Authors:
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E J Murphy; L A Horrocks |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1167 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1993 Apr |
Date Detail:
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Created Date: 1993-05-13 Completed Date: 1993-05-13 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 131-6 Citation Subset: IM |
Affiliation:
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Department of Medical Biochemistry, Ohio State University, Columbus. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Differentiation Choline / analysis Ethanolamine Ethanolamines / analysis Fatty Acids / analysis* Humans Neuroblastoma / chemistry* Phosphatidic Acids / analysis Phospholipids / chemistry* Plasmalogens / chemistry Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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NS-10165/NS/NINDS NIH HHS; NS-29441/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Ethanolamines; 0/Fatty Acids; 0/Phosphatidic Acids; 0/Phospholipids; 0/Plasmalogens; 141-43-5/Ethanolamine; 62-49-7/Choline |
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