Document Detail


Effects of different types of Helicobacter pylori on the gap junction intercellular communication in GES-1 cells.
MedLine Citation:
PMID:  21566279     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Objective To determine the effect of different types of Helicobacter pylori (H.pylori) on the gap junction intercellular communication (GJIC) in GES-1 cells, and investigate the types of H.pylori related to the dysfunction of GJIC. Methods Different types of H.pylori clinical strains were isolated and cultured, including the East Asian CagA(-)positive H.pylori (East Asian CagA(+)H.pylori), Western CagA(-)positive H.pylori (Western CagA(+)H.pylori), and the CagA(-)negative H.pylori (CagA(-)H.pylori). We co-cultured these H.pylori strains with GES-1 cells for 24 and 48 h, respectively. The control group was cultured without any H.pylori for 24 and 48 h. Change of the GJIC function in GES-1 cells was detected by the scrape-loading dye transfer (SLDT) technique. The cell proliferation of each group was examined by the methyl thiazolyl tetrazolium bromide (MTT) assay. Results The control group showed better GJIC function in the GES-1 cells, and the fluorescent dye migrated 4-5 rows to the adjacent cells at 24 and 48 h. Compared with the control group, the GJIC function of GES-1 cells in the CagA(-)H.pylori group decreased and the fluorescent dye migrated 3 rows to the adjacent cells. Compared with the control group and the CagA(-) H.pylori group, the GJIC function of GES-1 cells in the Western CagA(+)H.pylori group decreased and the fluorescent dye migrated 1-2 rows to the adjacent cells. The East Asian CagA(+)H.pylori group showed no GJIC function or weak GJIC function, and most of the fluorescent dye was confined to the area of scratched single row cells and only a few migrated 1-2 rows to the adjacent cells. Difference in the cell proliferation between the CagA(-)H.pylori group and the control group was not significant. The cell proliferation of the Western CagA(+)H.pylori group and the East Asian CagA(+)H.pylori group at bacterium-to-cell ratio of 100:1 and 200:1 was higher than that of the control group. The cell prolife-ration of the East Asian CagA(+)H.pylori group at bacterium-to-cell ratio of 400:1 was significantly lower than that of the control group at 48 h. Conclusion H.pylori can inhibit the GJIC function in GES-1 cells, which may be associated with CagA(+)H.pylori, especially with East Asian CagA(+)H.pylori. The effect of H.pylori on the proliferation of GES-1 cells is related to virulence factor CagA.
Authors:
Canxia Xu; Yulin Chen; Xiong Chen; Fen Wang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences     Volume:  36     ISSN:  1672-7347     ISO Abbreviation:  Zhong Nan Da Xue Xue Bao Yi Xue Ban     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-05-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101230586     Medline TA:  Zhong Nan Da Xue Xue Bao Yi Xue Ban     Country:  China    
Other Details:
Languages:  eng     Pagination:  294-300     Citation Subset:  IM    
Affiliation:
Department of Gastroenterology,Third Xiangya Hospital, Central South University, Changsha 410013, China xucanxia2000@hotmail.com.
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