| Effects of dietary supplementation with phytonutrients on vaccine-stimulated immunity against infection with Eimeria tenella. | |
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MedLine Citation:
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PMID: 21676547 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Two phytonutrient mixtures, VAC (carvacrol, cinnamaldehyde, and Capsicum oleoresin), and MC (Capsicum oleoresin and turmeric oleoresin), were evaluated for their effects on local and systemic immune responses following immunization of chickens with an Eimeria recombinant protein. Chickens were fed from hatch with a non-supplemented diet, or with diets supplemented with VAC or MC, animals were immunized subcutaneously with profilin at 7 days post-hatch, and orally challenged with virulent oocysts of Eimeria tenella at 17 days post-hatch. Immunity against infection was evaluated by (a) body weight, (b) fecal oocyst shedding, (c) anti-profilin serum antibody levels, (d) splenic lymphocyte profilin recall responses, (e) intestinal levels of cytokine gene transcripts, and (f) peripheral blood lymphocyte subpopulations. Profilin-immunized and Eimeria-infected chickens fed the VAC- or MC-supplemented diets had increased body weights compared with immunized and infected animals given the non-supplemented diet. However, fecal oocyst shedding was not affected in the experimental vs. control groups. Immunized chickens given the MC-supplemented diet displayed increased anti-profilin antibody levels and greater profilin-induced lymphocyte proliferation compared with non-supplemented controls. Anti-profilin antibody levels, but not cell proliferation, were increased in birds given the VAC-supplemented diet. Prior to Eimeria infection, immunized chickens fed the MC-supplemented diet had reduced levels of IFN-γ and IL-6 mRNAs, and increased expression of TNFSF15, compared with non-supplemented controls. Interestingly, post-infection levels of IFN-γ and IL-6 were increased, while IL-17F transcripts were decreased, with MC-supplementation. For VAC-supplemented diets, decreased levels of transcripts for IL-17F and TNFSF15 compared with non-supplemented controls were observed only in infected chickens. Finally, immunized chickens fed the MC-supplemented diet exhibited increased percentages of MHC class II(+), CD4(+), CD8(+), TCR1(+), and TCR2(+) lymphocytes compared with non-supplemented controls, while animals given the VAC containing diet only displayed an increase in K1(+) macrophages. In conclusion, this study documents the immune molecular and cellular changes following phytonutrient dietary supplementation that are relevant to protective immunity following recombinant protein vaccination against avian coccidiosis. |
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Authors:
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Sung Hyen Lee; Hyun S Lillehoj; Seung I Jang; Kyung Woo Lee; David Bravo; Erik P Lillehoj |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-10 |
Journal Detail:
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Title: Veterinary parasitology Volume: - ISSN: 1873-2550 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-6-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7602745 Medline TA: Vet Parasitol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Published by Elsevier B.V. |
Affiliation:
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Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service-U.S. Department of Agriculture, Beltsville, MD, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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