Document Detail

Effects of dietary retinoid and triglyceride on the lipid composition of rat liver stellate cells and stellate cell lipid droplets.
MedLine Citation:
PMID:  3241127     Owner:  NLM     Status:  MEDLINE    
Hepatic stellate cells store the majority of the liver's retinoid (vitamin A) reserves as retinyl esters in stellate cell lipid droplets. A study was conducted to explore the effects of differences in dietary retinoid and triglyceride intake on the composition of the stellate cell lipid droplets. Weanling rats were placed on one of five diets that differed in retinoid or triglyceride contents. The dietary groups were: 1) control (2.4 mg retinol (as retinyl acetate)/kg diet and 20.5% of the calories supplied by triglyceride (as peanut oil]; 2) low retinol (0.6 mg retinol/kg diet and control triglyceride levels); 3) high retinol (24 mg retinol/kg diet and control triglyceride levels); 4) low triglyceride (2.4 mg retinol/kg diet and 5% of the calories supplied by triglyceride); and 5) high triglyceride (2.4 mg retinol/kg diet and 45% of the calories supplied by triglyceride). Stellate cells were isolated using the pronase-collagenase method and stellate cell lipid droplets were isolated by differential centrifugation. The levels of retinoids and other lipids were measured by high performance liquid chromatography. The stellate cells from control rats contained 113 micrograms total lipid/10(6) cells. Control stellate cell lipid droplets had the following mean percent lipid composition: 39.5% retinyl ester; 31.7% triglyceride; 15.4% cholesteryl ester; 4.7% cholesterol; 6.3% phospholipids; and 2.4% free fatty acids. Both the concentration of stellate cell lipids and the composition of stellate cell lipid droplets were markedly altered by changes in dietary retinoid. The low and high retinol groups contained, respectively, 82 and 566 micrograms total lipid/10(6) cells, with retinyl ester representing, respectively, 13.6% and 65.4% of the lipid present in the stellate cell lipid droplets. Low and high triglyceride groups were similar to controls in both stellate cell lipid content and the composition of the stellate cell lipid droplets. These findings indicate that the composition of stellate cell lipid droplets is strongly regulated by dietary retinoid status but not by dietary triglyceride intake.
H Moriwaki; W S Blaner; R Piantedosi; D S Goodman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of lipid research     Volume:  29     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1988 Nov 
Date Detail:
Created Date:  1989-05-22     Completed Date:  1989-05-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1523-34     Citation Subset:  IM    
Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
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MeSH Terms
Cholesterol / analysis
Cholesterol Esters / analysis
Chromatography / methods
Chromatography, High Pressure Liquid
Fatty Acids, Nonesterified / analysis
Kupffer Cells / analysis
Lipid Metabolism*
Lipids / analysis
Liver / analysis,  drug effects,  metabolism*
Phospholipids / analysis
Rats, Inbred Strains
Retinoids / administration & dosage*
Triglycerides / administration & dosage*
Grant Support
Reg. No./Substance:
0/Cholesterol Esters; 0/Fatty Acids, Nonesterified; 0/Lipids; 0/Phospholipids; 0/Retinoids; 0/Triglycerides; 57-88-5/Cholesterol

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