Document Detail


Effects of dietary calcium restriction and acute exercise on the antioxidant enzyme system and oxidative stress in rat diaphragm.
MedLine Citation:
PMID:  14764436     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Calcium deficiency is considered to increase intracellular calcium level; thus the aim of the current study was to elucidate whether dietary calcium restriction enhanced exercise-induced oxidative stress in rat diaphragm. Twenty male Wistar rats were randomly assigned to either a control group or a group subjected to 1 mo of calcium restriction. In addition, each group was subsequently subdivided into rested or acutely exercised group. Dietary calcium restriction significantly (P < 0.05) upregulated the activities of manganese-superoxide dismutase (Mn-SOD), copper-zinc-superoxide dismutase (Cu-Zn-SOD), and glutathione peroxidase (Gpx) but not catalase. Acute exercise, in addition to calcium restriction, decreased both SOD isoenzymes in the diaphragm of calcium-restricted rats (P < 0.05). On the other hand, calcium restriction resulted in increased Gpx mRNA expression (P < 0.05). In control rats, acute exercise significantly (P < 0.05) increased the expressions of both SOD mRNAs, whereas in the calcium-restricted rats, it increased that of Mn-SOD mRNA (P < 0.05) but decreased that of Gpx mRNA (P < 0.05). Furthermore, reactive carbonyl derivative, a marker of protein oxidation, was significantly greater in the calcium-restricted rats than in the control rats after acute exercise (P < 0.05). The results suggest that antioxidant enzymes in rat diaphragm were upregulated in response to an increased oxidative stress by dietary calcium restriction but that upregulation is not enough to cope with exercise-induced further increase of oxidative stress.
Authors:
Masayuki Itoh; Shuji Oh-Ishi; Hidekazu Hatao; Christiaan Leeuwenburgh; Colin Selman; Hideki Ohno; Takako Kizaki; Hiroyuki Nakamura; Takeshi Matsuoka
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Publication Detail:
Type:  Journal Article     Date:  2004-02-05
Journal Detail:
Title:  American journal of physiology. Regulatory, integrative and comparative physiology     Volume:  287     ISSN:  0363-6119     ISO Abbreviation:  Am. J. Physiol. Regul. Integr. Comp. Physiol.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-06-11     Completed Date:  2004-07-13     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100901230     Medline TA:  Am J Physiol Regul Integr Comp Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  R33-8     Citation Subset:  IM    
Affiliation:
Fifth Dept. of Internal Medicine, Tokyo Medical Univ., 3-20-1, Chuou, Ami, Inashiki, Ibaraki 300-0395, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antioxidants / metabolism*
Body Weight / physiology
Bone Density / drug effects
Calcium / deficiency*
Calcium, Dietary*
Catalase / metabolism
DNA Primers
Diaphragm / enzymology,  physiology*
Diet*
Glutathione Peroxidase / metabolism
Male
Muscle Proteins / metabolism
Organ Size / physiology
Oxidative Stress / physiology*
Physical Exertion / physiology*
RNA, Messenger / biosynthesis
Rats
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Superoxide Dismutase / metabolism
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Calcium, Dietary; 0/DNA Primers; 0/Muscle Proteins; 0/RNA, Messenger; 7440-70-2/Calcium; EC 1.11.1.6/Catalase; EC 1.11.1.9/Glutathione Peroxidase; EC 1.15.1.1/Superoxide Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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