Document Detail

Effects of dietary calcium on adipocyte lipid metabolism and body weight regulation in energy-restricted aP2-agouti transgenic mice.
MedLine Citation:
PMID:  11156940     Owner:  NLM     Status:  MEDLINE    
We have demonstrated previously a regulatory role for intracellular Ca2+ ([Ca2+]i) in adipocyte lipogenesis and lipolysis and have recently reported that 1,25-(OH)2-D increases adipocyte [Ca2+]i, which causes increased lipogenesis and decreased lipolysis. We have now tested the hypothesis that suppressing 1,25-(OH)2-D by increasing dietary calcium will suppress adipocyte [Ca2+]i, thereby facilitating weight loss by stimulating lipolysis and inhibiting lipogenesis in calorically (Kcal)-restricted (70% of ad lib) aP2-agouti transgenic (aP2-a) mice. Mice (aP2-a) exhibiting a pattern of obesity gene expression similar to humans were fed a low-Ca (0.4%)/high-fat/high-sucrose diet for six weeks, resulting in a 27% and twofold increase in body weight and total fat pad mass, respectively, with a twofold increase in adipocyte [Ca2+]i pad lib or Kcal-restricted (70% of ad lib) on this diet either unsupplemented (basal) or with 25% or 50% of the protein replaced by non-fat dry milk (medium or high) dairy or supplemented with CaCO3 to 1.2% Ca for six weeks. Adipocyte [Ca2+]i was unaffected by Kcal restriction but was reduced markedly by all three high Ca diets (290 vs. 130 nM, p2+]i and thereby reduce energy storage and increase thermogenesis during Kcal restriction.
H Shi; D Dirienzo; M B Zemel
Publication Detail:
Type:  Journal Article     Date:  2000-12-08
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  15     ISSN:  0892-6638     ISO Abbreviation:  FASEB J.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-03-22     Revised Date:  2012-02-15    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  291-3     Citation Subset:  IM    
The University of Tennessee, Knoxville, TN 37996-1900, USA.
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MeSH Terms
Adipocytes / drug effects,  metabolism*
Adipose Tissue / anatomy & histology,  cytology,  physiology*
Body Temperature Regulation
Body Weight* / genetics
Calcitriol / pharmacology
Calcium, Dietary / pharmacology*
Diet, Reducing
Energy Metabolism* / genetics
Ion Channels
Lipid Metabolism*
Membrane Transport Proteins*
Mice, Inbred Strains
Mice, Transgenic
Mitochondrial Proteins*
Models, Biological
Obesity / genetics,  physiopathology*
Proteins / genetics,  metabolism
Reg. No./Substance:
0/Calcium, Dietary; 0/Ion Channels; 0/Membrane Transport Proteins; 0/Mitochondrial Proteins; 0/Proteins; 0/mitochondrial uncoupling protein 2; 32222-06-3/Calcitriol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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