Document Detail

Effects of diet-induced hypercholesterolemia on amyloid accumulation in ovariectomized mice.
MedLine Citation:
PMID:  23151792     Owner:  NLM     Status:  In-Data-Review    
A central hypothesis in the study of Alzheimer's disease (AD) is the accumulation and aggregation of beta-amyloid peptide (A beta). Recent epidemiological studies suggest that patients with elevated cholesterol and decreased estrogen levels are more susceptible to AD through A beta accumulation. To test the above hypothesis, we used ovariectomized with diet-induced hypercholesterolemia (OVX) and hypercholesterolemia (HCL) diet alone mouse models. HPLC analysis reveals the presence of beta amyloid in the OVX and HCL mice brain. Congo red staining analysis revealed the extent of amyloid deposition in OVX and hypercholesterolemia mice brain. Overall, A beta levels were higher in OVX mice than in HCL. Secondly, estrogen receptors alpha (ER alpha) were assessed by immunohistochemistry and this suggested that there was a decreased expression of ER alpha in OVX animals when compared to hypercholesterolemic animals. A beta was quantified by Western blot and ELISA analysis. Overall, A beta levels were higher in OVX mice than in HCL mice. Our experimental results suggested that OVX animals were more susceptible to AD with significant increase in A beta peptide.
V Kaliyamurthi; V Thanigavelan; G Victor Rajamanickam
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biosciences     Volume:  37     ISSN:  0973-7138     ISO Abbreviation:  J. Biosci.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100809     Medline TA:  J Biosci     Country:  India    
Other Details:
Languages:  eng     Pagination:  1017-27     Citation Subset:  IM    
Department of Bio-Organic and Neurochemistry Laboratory, Central Leather Research Institute, Chennai 600 020,
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