Document Detail

Effects of dibutyryl cAMP and bromodeoxyuridine on expression of N-acetylglucosaminyltransferases III and V in GOTO neuroblastoma cells.
MedLine Citation:
PMID:  8748156     Owner:  NLM     Status:  MEDLINE    
The sugar chain structures of the cell surface change dramatically during cellular differentiation. A human neuroblastoma cell line, GOTO, is known to differentiate into neuronal cells and Schwannian cell-like cells on treatments with dibutyryl cAMP and bromodeoxyuridine, respectively. We have examined the expression of UDP-N-acetylglucosamine: beta-D-mannoside beta-1,4N-acetylglucosaminyltransferase III (GnT-III: EC and UDP-N-acetylglucosamine: alpha-6-D-mannoside beta-1,6N-acetylglucosaminyltransferase V (GnT-V: EC, two major branch forming enzymes in N-glycan synthesis, in GOTO cells on two distinct directions of differentiation. In neuronal cell differentiation, GnT-III activity showed a slight increase during initial treatment with Bt2cAMP for 4 days and decreased drastically after the fourth day, but the mRNA level of GnT-III did not show a decrease but in fact a slight increase. GnT-V activity increased to approximately two- to three-fold the initial level with increasing mRNA level after 8 days, and lectin blot analysis showed an increase in reactivity to Datsura stramonium (DSA) of the immunoprecipitated neural cell adhesion molecule (NCAM). In Schwannian cell differentiation, the activity and mRNA level of GnT-III showed no significant change on treatment with BrdU. GnT-V activity also showed no change in spite of the gradual increase in the mRNA level. These results suggest that the activation of GnT-V during neuronal cell differentiation of GOTO cells might be a specific change for branch formation in N-glycans, and this affects the sugar chain structures of some glycoproteins such as NCAM.
Y Ihara; A Nishikawa; N Taniguchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Glycoconjugate journal     Volume:  12     ISSN:  0282-0080     ISO Abbreviation:  Glycoconj. J.     Publication Date:  1995 Dec 
Date Detail:
Created Date:  1996-11-15     Completed Date:  1996-11-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8603310     Medline TA:  Glycoconj J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  787-94     Citation Subset:  IM    
Department of Biochemistry, Osaka University Medical School, Japan.
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MeSH Terms
Bromodeoxyuridine / pharmacology
Bucladesine / pharmacology*
Carbohydrate Sequence
Cell Differentiation / drug effects
Cell Division / drug effects
Molecular Sequence Data
N-Acetylglucosaminyltransferases / biosynthesis*
Neural Cell Adhesion Molecules / analysis
Neurons / drug effects*,  enzymology
RNA, Messenger / biosynthesis
Schwann Cells / drug effects*,  enzymology
Tumor Cells, Cultured
Reg. No./Substance:
0/Lectins; 0/Neural Cell Adhesion Molecules; 0/RNA, Messenger; 362-74-3/Bucladesine; 59-14-3/Bromodeoxyuridine; EC 2.4.1.-/N-Acetylglucosaminyltransferases; EC,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase; EC,3-mannosylglycoprotein beta-1,4-N-acetylglucosaminyltransferase

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