| Effects of dibutyryl cAMP and bromodeoxyuridine on expression of N-acetylglucosaminyltransferases III and V in GOTO neuroblastoma cells. | |
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MedLine Citation:
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PMID: 8748156 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The sugar chain structures of the cell surface change dramatically during cellular differentiation. A human neuroblastoma cell line, GOTO, is known to differentiate into neuronal cells and Schwannian cell-like cells on treatments with dibutyryl cAMP and bromodeoxyuridine, respectively. We have examined the expression of UDP-N-acetylglucosamine: beta-D-mannoside beta-1,4N-acetylglucosaminyltransferase III (GnT-III: EC 2.4.1.144) and UDP-N-acetylglucosamine: alpha-6-D-mannoside beta-1,6N-acetylglucosaminyltransferase V (GnT-V: EC 2.4.1.155), two major branch forming enzymes in N-glycan synthesis, in GOTO cells on two distinct directions of differentiation. In neuronal cell differentiation, GnT-III activity showed a slight increase during initial treatment with Bt2cAMP for 4 days and decreased drastically after the fourth day, but the mRNA level of GnT-III did not show a decrease but in fact a slight increase. GnT-V activity increased to approximately two- to three-fold the initial level with increasing mRNA level after 8 days, and lectin blot analysis showed an increase in reactivity to Datsura stramonium (DSA) of the immunoprecipitated neural cell adhesion molecule (NCAM). In Schwannian cell differentiation, the activity and mRNA level of GnT-III showed no significant change on treatment with BrdU. GnT-V activity also showed no change in spite of the gradual increase in the mRNA level. These results suggest that the activation of GnT-V during neuronal cell differentiation of GOTO cells might be a specific change for branch formation in N-glycans, and this affects the sugar chain structures of some glycoproteins such as NCAM. |
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Authors:
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Y Ihara; A Nishikawa; N Taniguchi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Glycoconjugate journal Volume: 12 ISSN: 0282-0080 ISO Abbreviation: Glycoconj. J. Publication Date: 1995 Dec |
Date Detail:
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Created Date: 1996-11-15 Completed Date: 1996-11-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8603310 Medline TA: Glycoconj J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 787-94 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Osaka University Medical School, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bromodeoxyuridine
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pharmacology Bucladesine / pharmacology* Carbohydrate Sequence Cell Differentiation / drug effects Cell Division / drug effects Humans Lectins Molecular Sequence Data N-Acetylglucosaminyltransferases / biosynthesis* Neural Cell Adhesion Molecules / analysis Neuroblastoma Neurons / drug effects*, enzymology RNA, Messenger / biosynthesis Schwann Cells / drug effects*, enzymology Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Lectins; 0/Neural Cell Adhesion Molecules; 0/RNA, Messenger; 362-74-3/Bucladesine; 59-14-3/Bromodeoxyuridine; EC 2.4.1.-/N-Acetylglucosaminyltransferases; EC 2.4.1.144/beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase; EC 2.4.1.145/alpha-1,3-mannosylglycoprotein beta-1,4-N-acetylglucosaminyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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