Document Detail


Effects of dextranation on the pharmacokinetics of short peptides. A PET study on mEGF.
MedLine Citation:
PMID:  10563762     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of dextranation on the biodistribution of mouse epidermal growth factor (mEGF, 6 kDa) were assessed. By reductive amination, mEGF was coupled to 13 and 46 kDa dextran. The two dextranated conjugates and free mEGF were labeled with the positron-emitting nuclide (76)Br (T(1/2) = 16 h). After intravenous administration to Sprague Dawley rats, the radioactivity biodistribution was evaluated by positron emission tomography (PET) and by measurements of dissected tissues. The dextranation prolonged the retention time in blood, especially when the dextran chain was long. [(76)Br]mEGF-dextran conjugates were shown to have significantly, more than 5 times, lower kidney accumulation than the nonconjugated [(76)Br]mEGF. In conclusion, dextranation affects the biodistribution of mEGF in vivo giving a prolonged circulation time, a decreased uptake in kidney, and an increased spleen accumulation.
Authors:
Q Zhao; V Tolmachev; J Carlsson; H Lundqvist; J Sundin; J C Janson; A Sundin
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioconjugate chemistry     Volume:  10     ISSN:  1043-1802     ISO Abbreviation:  Bioconjug. Chem.     Publication Date:    1999 Nov-Dec
Date Detail:
Created Date:  2000-01-06     Completed Date:  2000-01-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9010319     Medline TA:  Bioconjug Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  938-46     Citation Subset:  IM    
Affiliation:
Biomedical Radiation Sciences, Box 535, Uppsala University, S-751 21 Uppsala, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bromine Radioisotopes
Chromatography, High Pressure Liquid
Dextrans / chemistry*
Epidermal Growth Factor / analysis,  chemistry*,  pharmacokinetics*
Kidney / metabolism
Kinetics
Liver / metabolism
Male
Mice
Rats
Rats, Sprague-Dawley
Spleen / metabolism
Tissue Distribution
Tomography, Emission-Computed
Chemical
Reg. No./Substance:
0/Bromine Radioisotopes; 62229-50-9/Epidermal Growth Factor; 9004-54-0/Dextrans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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