Document Detail

Effects of dexamethasone on mucin gene expression in cultured human nasal epithelial cells.
MedLine Citation:
PMID:  12172258     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To clarify 1) which mucin gene expression is influenced by glucocorticoid and 2) whether glucocorticoid influences steady-state mucin expression or mucin gene expression induced by lipopolysaccharide. METHODS: Dissociated cells obtained from nasal polyps were cultured on a collagen gel substrate in an air-liquid interface. Dexamethasone was added to the culture medium in the steady-state and prior to the stimulation by LPS. RNAs were extracted from culture cells, and semiquantitative reverse transcriptase-polymerase chain reaction was performed for MUC2, MUC5AC, MUC5B, MUC8, and beta-actin. RESULTS: Dexamethasone did not influence steady-state messenger RNA levels of either mucin gene. Dexamethasone suppressed lipopolysaccharide-induced messenger RNA levels of MUC8. CONCLUSION: Glucocorticoid does not influence steady-state mucin gene expression but suppresses mucin gene expression induced by secretagogues such as lipopolysaccharide.
Hajime Ishinaga; Kazuhiko Takeuchi; Chikako Kishioka; Mayuko Yagawa; Yuichi Majima
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Laryngoscope     Volume:  112     ISSN:  0023-852X     ISO Abbreviation:  Laryngoscope     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-08-12     Completed Date:  2002-09-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8607378     Medline TA:  Laryngoscope     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1436-40     Citation Subset:  IM    
Department of Otorhinolaryngology, Mie University School of Medicine, Tsu, Mie, Japan.
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MeSH Terms
Cells, Cultured
Dexamethasone / pharmacology*
Gene Expression Regulation / drug effects*
Glucocorticoids / pharmacology*
Lipopolysaccharides / pharmacology
Mucins / drug effects*,  genetics*
Nasal Mucosa / cytology*,  drug effects*
Reg. No./Substance:
0/Glucocorticoids; 0/Lipopolysaccharides; 0/Mucins; 50-02-2/Dexamethasone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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