| Effects of dexamethasone on mucin gene expression in cultured human nasal epithelial cells. | |
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MedLine Citation:
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PMID: 12172258 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To clarify 1) which mucin gene expression is influenced by glucocorticoid and 2) whether glucocorticoid influences steady-state mucin expression or mucin gene expression induced by lipopolysaccharide. METHODS: Dissociated cells obtained from nasal polyps were cultured on a collagen gel substrate in an air-liquid interface. Dexamethasone was added to the culture medium in the steady-state and prior to the stimulation by LPS. RNAs were extracted from culture cells, and semiquantitative reverse transcriptase-polymerase chain reaction was performed for MUC2, MUC5AC, MUC5B, MUC8, and beta-actin. RESULTS: Dexamethasone did not influence steady-state messenger RNA levels of either mucin gene. Dexamethasone suppressed lipopolysaccharide-induced messenger RNA levels of MUC8. CONCLUSION: Glucocorticoid does not influence steady-state mucin gene expression but suppresses mucin gene expression induced by secretagogues such as lipopolysaccharide. |
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Authors:
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Hajime Ishinaga; Kazuhiko Takeuchi; Chikako Kishioka; Mayuko Yagawa; Yuichi Majima |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Laryngoscope Volume: 112 ISSN: 0023-852X ISO Abbreviation: Laryngoscope Publication Date: 2002 Aug |
Date Detail:
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Created Date: 2002-08-12 Completed Date: 2002-09-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8607378 Medline TA: Laryngoscope Country: United States |
Other Details:
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Languages: eng Pagination: 1436-40 Citation Subset: IM |
Affiliation:
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Department of Otorhinolaryngology, Mie University School of Medicine, Tsu, Mie, Japan. hajime@clin.med.mie-u.ac.jp |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cells, Cultured Dexamethasone / pharmacology* Gene Expression Regulation / drug effects* Glucocorticoids / pharmacology* Humans Lipopolysaccharides / pharmacology Mucins / drug effects*, genetics* Nasal Mucosa / cytology*, drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Glucocorticoids; 0/Lipopolysaccharides; 0/Mucins; 50-02-2/Dexamethasone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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