| Effects of dentin bonding agents on the cell cycle of fibroblasts. | |
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MedLine Citation:
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PMID: 19166789 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to evaluate the effects of 3 dentin bonding agents on cell survival and proliferation and on cell cycle progression of cultured cells. The experiments were performed on RPC-C2A and L929 cells. Specimens of the 3 dentin bonding agents (Clearfil Tri-S, AdheSE, and XP BOND) were placed in culture medium, and the extraction media were applied to cells as experimental material. The effect of the bonding materials on cell survival and proliferation was assessed by a modified sulforhodamine B staining assay, and the effect on DNA synthesis was assessed by bromodeoxyuridine uptake. Flow cytometry was used for cell cycle analysis. Cell viability and proliferation decreased in a dose-dependent manner after exposure of cells to the tested materials. XP BOND expressed the highest activity of all tested bonding agents (P < .05). The self-etch bonding agents tested did not produce any significant effects on cell cycle distribution. However, exposure of cells to the total-etch agent XP BOND induced a G(2)-phase arrest in both cell lines, and this effect was more evident in L929 cells than in RPC-C2A cells. |
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Authors:
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Elisabeth A Koulaouzidou; Konstantinos T Papazisis; Efi Yiannaki; Georgios Palaghias; Maria Helvatjoglu-Antoniades |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of endodontics Volume: 35 ISSN: 1878-3554 ISO Abbreviation: J Endod Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-01-26 Completed Date: 2009-05-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7511484 Medline TA: J Endod Country: United States |
Other Details:
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Languages: eng Pagination: 275-9 Citation Subset: D |
Affiliation:
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Department of Operative Dentistry, Dental School, Aristotle University, Thessaloniki, Greece. koulaouz@dent.auth.gr |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acrylic Resins
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toxicity Animals Cell Cycle / drug effects* Cell Proliferation / drug effects Cell Survival / drug effects Cells, Cultured DNA / biosynthesis Dental Pulp / cytology, drug effects* Dentin-Bonding Agents / toxicity* Fibroblasts / drug effects* L Cells (Cell Line) Materials Testing Mice Rats Resin Cements / toxicity* |
| Chemical | |
Reg. No./Substance:
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0/Acrylic Resins; 0/AdheSE cement; 0/Clearfil Tri-S Bond; 0/Dentin-Bonding Agents; 0/Resin Cements; 0/XP-Bond; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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