Document Detail


Effects of decorin and biglycan on human airway smooth muscle cell proliferation and apoptosis.
MedLine Citation:
PMID:  18245265     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Proteoglycans (PG) are altered in the asthmatic airway wall. Because PGs are known to affect cell proliferation and apoptosis, we hypothesized that alterations in PG might influence the airway smooth muscle (ASM) hyperplasia observed in the asthmatic airway. Human ASM cells were seeded on plastic or plates coated with decorin (Dcn), biglycan (Bgn), or collagen type I (Col I) (1, 3, and 10 microg/ml). Cells were stimulated with platelet-derived growth factor (PDGF), and cell number was assessed at 0, 48, and 96 h. Cell proliferation was measured by bromodeoxyuridine (BrdU) incorporation and apoptosis by annexin V and propidium iodide staining at 48 h post-PDGF stimulation. A significant decrease in cell number was observed with cells seeded on Dcn (10 microg/ml) at 0, 48, and 96 h (P < 0.01). Dcn induced both decreases in BrdU incorporation and increases in annexin V staining (P < 0.05). Bgn decreased cell number at time 0 only (P < 0.05) and affected neither proliferation nor apoptosis. Col I (10 mug/ml) caused a significant increase in cell number at 48 and 96 h (P < 0.01). Adding exogenous Dcn (1-30 microg/ml) to the medium had no effect on cell number. Exposing Dcn-coated matrices to chondroitinase ABC, an enzyme that degrades glycosaminoglycan side chains, reversed the Dcn-induced decrease in cell number. These studies demonstrate that different PGs have variable effects on ASM cell proliferation and apoptosis. Recently described decreases in Dcn in the asthmatic airway wall could potentially permit more exuberant ASM growth.
Authors:
Michelle L D'Antoni; Chiara Torregiani; Pasquale Ferraro; Marie-Claire Michoud; Bruce Mazer; James G Martin; Mara S Ludwig
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-01
Journal Detail:
Title:  American journal of physiology. Lung cellular and molecular physiology     Volume:  294     ISSN:  1040-0605     ISO Abbreviation:  Am. J. Physiol. Lung Cell Mol. Physiol.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-08     Completed Date:  2008-06-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901229     Medline TA:  Am J Physiol Lung Cell Mol Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  L764-71     Citation Subset:  IM    
Affiliation:
Meakins Christie Laboratories, McGill University, Montreal, Quebec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects*
Asthma / physiopathology
Cell Count
Cell Culture Techniques
Cell Division / drug effects*
Culture Media
Extracellular Matrix Proteins / pharmacology*
Flow Cytometry
Humans
Muscle, Smooth / cytology,  drug effects,  physiology*,  physiopathology
Proteoglycans / pharmacology*
Respiratory System / drug effects*
Chemical
Reg. No./Substance:
0/Culture Media; 0/Extracellular Matrix Proteins; 0/Proteoglycans; 0/decorin; 123939-84-4/biglycan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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