Document Detail


Effects of cytochalasins B and D on the fertilization of zebrafish (Brachydanio) eggs.
MedLine Citation:
PMID:  3392518     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of selected concentrations of cytochalasins B (1-10 micrograms/ml; CB) and D (10, 50 micrograms/ml; CD) on the morphology and fertilization of zebra danio (Brachydanio) eggs were studied primarily with light and scanning electron microscopy. Eggs pretreated with either CB (10 micrograms/ml) or CD (10, 50 micrograms/ml) prepared in Fish Ringer's solution-0.5% DMSO showed a flattened shape, alterations in the form of surface microplicae and microvilli, and occasional spontaneous exocytosis of cortical granules. All eggs preincubated in either CB or CD were activated upon transfer to tap water, showing cortical granule exocytosis, elevation of the chorion, and formation of a fertilization cone. When eggs were pretreated for 5 minutes with 1-5 micrograms/ml CB or 10 micrograms/ml CD and inseminated, they incorporated the fertilizing sperm and typically developed to the two-cell stage. A single sperm cell attached to and fused with the sperm entry site microvilli but failed to enter the cytoplasm in eggs preincubated with 10 micrograms/ml CB. Eggs that were immersed continuously in either CB (10 micrograms/ml) or CD (50 micrograms/ml) 15 seconds after insemination also failed to incorporate the fertilizing sperm. Treatment of eggs after insemination with CD (10 micrograms/ml), however, did not prevent sperm cell incorporation or fertilization cone formation. Our drug data suggest the presence of actin-containing filaments in the danio egg before and following fertilization. These filaments appear to play a role in maintaining the shape of the egg cell and its surface specializations and in the incorporation of the fertilizing sperm. The fertilization cone appears to form independently of actin polymerization.
Authors:
J S Wolenski; N H Hart
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of experimental zoology     Volume:  246     ISSN:  0022-104X     ISO Abbreviation:  J. Exp. Zool.     Publication Date:  1988 May 
Date Detail:
Created Date:  1988-08-23     Completed Date:  1988-08-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375365     Medline TA:  J Exp Zool     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  202-15     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Rutgers University, New Brunswick, New Jersey 08903.
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MeSH Terms
Descriptor/Qualifier:
Actins
Animals
Cyprinidae / physiology*
Cytochalasin B / pharmacology*
Cytochalasin D
Cytochalasins / pharmacology*
Exocytosis
Female
Fertilization / drug effects*
Male
Microfilaments
Microscopy, Electron
Microscopy, Electron, Scanning
Microvilli / drug effects
Ovum / drug effects*,  ultrastructure
Parthenogenesis / drug effects
Sperm-Ovum Interactions / drug effects
Zebrafish / physiology*
Grant Support
ID/Acronym/Agency:
HD 17647/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 0/Cytochalasins; 14930-96-2/Cytochalasin B; 22144-77-0/Cytochalasin D

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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