Document Detail


Effects of cyclooxygenase-2 non-selective and selective inhibitors on proliferation inhibition and apoptosis induction of esophageal squamous carcinoma cells.
MedLine Citation:
PMID:  18564172     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of this study is to investigate the effects of aspirin and nimesulide on cell proliferation, apoptosis and its potential mechanisms in EC9706 and EC109 esophageal squamous carcinoma cells. EC9706 and EC109 cells were incubated with varying concentrations of aspirin and nimesulide, and the effects on cell proliferation and apoptosis were monitored by 3-(4,5-dimethyl-thiazol-2yl)-2,5-diphenyltetrazolium bromide and flow cytometry. Reverse transcription-polymerase chain reaction and Western blot assays were used to investigate expression of Bcl-2 and Bax. Prostaglandin E2 production was measured by enzyme linked immunosorbent assay. Pretreatment with aspirin and nimesulide inhibited EC9706 and EC109 cell growth in a time and dose-dependent manner, accompanied with a decrease of prostaglandin E2 production. In EC9706 cells, the mechanism of aspirin and nimesulide induced growth inhibition was a consequence of cell cycle arrest at the G(0)/G(1) check point. In EC109 cells, growth arrest was by induction of apoptosis, associated with downregulation of Bcl-2, but not Bax. In conclusion, aspirin and nimesulide could inhibit cell proliferation and induce apoptosis in esophageal squamous carcinoma cells. Cyclooxygenase-2 inhibitor may be a promising therapeutic agent for human esophageal squamous cell carcinoma.
Authors:
P Li; S T Zhang; Z L Yu; Y D Wu; X Liu; C M Xu; C H Cho
Publication Detail:
Type:  Journal Article     Date:  2008-06-17
Journal Detail:
Title:  Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus / I.S.D.E     Volume:  22     ISSN:  1442-2050     ISO Abbreviation:  Dis. Esophagus     Publication Date:  2009  
Date Detail:
Created Date:  2009-02-06     Completed Date:  2009-06-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8809160     Medline TA:  Dis Esophagus     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  21-31     Citation Subset:  IM    
Affiliation:
Department of Digestive Diseases, Beijing Friendship Hospital, Capital Medical University; Beijing, China.
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MeSH Terms
Descriptor/Qualifier:
Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
Apoptosis / physiology
Aspirin / pharmacology*
Blotting, Western
Carcinoma, Squamous Cell / physiopathology*
Cell Line, Tumor
Cell Proliferation / drug effects*
Dinoprostone / metabolism
Esophageal Neoplasms / physiopathology*
Humans
In Situ Nick-End Labeling
Proto-Oncogene Proteins c-bcl-2 / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Sulfonamides / pharmacology*
bcl-2-Associated X Protein / metabolism*
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Proto-Oncogene Proteins c-bcl-2; 0/Sulfonamides; 0/bcl-2-Associated X Protein; 363-24-6/Dinoprostone; 50-78-2/Aspirin; 51803-78-2/nimesulide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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