| Effects of cyclooxygenase-2 on human esophageal squamous cell carcinoma. | |
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MedLine Citation:
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PMID: 22147962 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To study the relationship between the cyclooxygenase (COX)-2 gene and the proliferation and apoptosis of esophageal squamous carcinoma EC109 cells. METHODS: The techniques of RNA interference (RNAi) and cell transfection, as well as the levels of oncogenicity in nude mice, were used to study the role of COX-2 in the esophageal squamous carcinoma cell (ESCC) line EC109. Following RNAi and transfection, Western blotting analysis was used to determine the expression of the COX-2 protein. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) reduction assay was used to evaluate cell growth, and flow cytometry was used to detect cell apoptosis. RESULTS: Western blotting analysis demonstrated that COX-2 expression was significantly reduced in EC109 cells treated with COX-2-specific short interfering RNA (siRNA) but was increased in EC109 cells transfected with COX-2. Furthermore, COX-2 siRNA treatment inhibited cell proliferation (P < 0.01) and induced apoptosis in EC109 cells, as determined by an MTT assay and by flow cytometry, respectively. In contrast, transfected COX-2 led to increased cell proliferation (P < 0.05) and decreased apoptosis in EC109 cells. In addition, combination treatment of cells with COX-2 siRNA and aspirin had a synergistic effect (P < 0.01). For experiments measuring tumorigenicity, xenograft tumors of a greater volume and weight were found in the COX-2 group compared with other groups (P < 0.05). A large dose of aspirin inhibited tumor growth in nude mice effectively (P < 0.05), and the rate of tumor suppression was 51.8% in the high-dose aspirin group. CONCLUSION: COX-2 plays a very critical role in ESCC carcinogenesis, and COX-2 siRNA combined with aspirin has the potential to be an anticancer therapy for the treatment of ESCC. |
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Authors:
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Li Zhang; Yong-Dong Wu; Peng Li; Jun Tu; Ying-Lin Niu; Cai-Min Xu; Shu-Tian Zhang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: World journal of gastroenterology : WJG Volume: 17 ISSN: 1007-9327 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-12-07 Completed Date: 2012-02-08 Revised Date: 2013-05-29 |
Medline Journal Info:
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Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China |
Other Details:
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Languages: eng Pagination: 4572-80 Citation Subset: IM |
Affiliation:
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Department of Gastroenterology, Beijing Friendship Hospital Affiliated with the Capital Medical University, Beijing 100050, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects Aspirin / pharmacology Carcinoma, Squamous Cell / enzymology*, genetics, pathology, physiopathology Cell Line, Tumor Cell Proliferation / drug effects Cyclooxygenase 2 / genetics, metabolism* Cyclooxygenase Inhibitors / pharmacology Esophageal Neoplasms / enzymology*, genetics, pathology, physiopathology Gene Expression Regulation, Enzymologic / drug effects Gene Expression Regulation, Neoplastic / drug effects Humans Male Mice Mice, Inbred BALB C Mice, Nude Neoplasm Transplantation RNA Interference RNA, Small Interfering / genetics, metabolism Transfection |
| Chemical | |
Reg. No./Substance:
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0/Cyclooxygenase Inhibitors; 0/RNA, Small Interfering; 50-78-2/Aspirin; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/PTGS2 protein, human |
| Comments/Corrections | |
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