Document Detail


Effects of cyclooxygenase-2 on human esophageal squamous cell carcinoma.
MedLine Citation:
PMID:  22147962     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To study the relationship between the cyclooxygenase (COX)-2 gene and the proliferation and apoptosis of esophageal squamous carcinoma EC109 cells.
METHODS: The techniques of RNA interference (RNAi) and cell transfection, as well as the levels of oncogenicity in nude mice, were used to study the role of COX-2 in the esophageal squamous carcinoma cell (ESCC) line EC109. Following RNAi and transfection, Western blotting analysis was used to determine the expression of the COX-2 protein. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) reduction assay was used to evaluate cell growth, and flow cytometry was used to detect cell apoptosis.
RESULTS: Western blotting analysis demonstrated that COX-2 expression was significantly reduced in EC109 cells treated with COX-2-specific short interfering RNA (siRNA) but was increased in EC109 cells transfected with COX-2. Furthermore, COX-2 siRNA treatment inhibited cell proliferation (P < 0.01) and induced apoptosis in EC109 cells, as determined by an MTT assay and by flow cytometry, respectively. In contrast, transfected COX-2 led to increased cell proliferation (P < 0.05) and decreased apoptosis in EC109 cells. In addition, combination treatment of cells with COX-2 siRNA and aspirin had a synergistic effect (P < 0.01). For experiments measuring tumorigenicity, xenograft tumors of a greater volume and weight were found in the COX-2 group compared with other groups (P < 0.05). A large dose of aspirin inhibited tumor growth in nude mice effectively (P < 0.05), and the rate of tumor suppression was 51.8% in the high-dose aspirin group.
CONCLUSION: COX-2 plays a very critical role in ESCC carcinogenesis, and COX-2 siRNA combined with aspirin has the potential to be an anticancer therapy for the treatment of ESCC.
Authors:
Li Zhang; Yong-Dong Wu; Peng Li; Jun Tu; Ying-Lin Niu; Cai-Min Xu; Shu-Tian Zhang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  17     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-12-07     Completed Date:  2012-02-08     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  4572-80     Citation Subset:  IM    
Affiliation:
Department of Gastroenterology, Beijing Friendship Hospital Affiliated with the Capital Medical University, Beijing 100050, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects
Aspirin / pharmacology
Carcinoma, Squamous Cell / enzymology*,  genetics,  pathology,  physiopathology
Cell Line, Tumor
Cell Proliferation / drug effects
Cyclooxygenase 2 / genetics,  metabolism*
Cyclooxygenase Inhibitors / pharmacology
Esophageal Neoplasms / enzymology*,  genetics,  pathology,  physiopathology
Gene Expression Regulation, Enzymologic / drug effects
Gene Expression Regulation, Neoplastic / drug effects
Humans
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasm Transplantation
RNA Interference
RNA, Small Interfering / genetics,  metabolism
Transfection
Chemical
Reg. No./Substance:
0/Cyclooxygenase Inhibitors; 0/RNA, Small Interfering; 50-78-2/Aspirin; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/PTGS2 protein, human
Comments/Corrections

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