Document Detail


Effects of cycloheximide on parthenogenetic development of pig oocytes activated by ultrasound treatment.
MedLine Citation:
PMID:  18635923     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study was carried out to examine the parthenogenetic development of pig oocytes treated with different concentrations of cycloheximide for different durations following activation by ultrasound stimulation. When oocytes were treated with 10 microg/ml cycloheximide for different durations, the blastocyst formation rate of oocytes treated for 5 h was significantly (P<0.05) higher than those of oocytes treated for 0-2 h. The blastocyst formation rate of oocytes treated with 10 microg/ml cycloheximide for 5 h was significantly (P<0.05) higher than those of oocytes treated with 0-5 or 15-20 microg/ml cycloheximide for the same duration. When oocytes were treated with different concentrations of cycloheximide for 2 h, however, the blastocyst formation rate of oocytes treated with 40 microg/ml cycloheximide was significantly (P<0.05) higher than those of oocytes treated with 0-10 or 50 microg/ml cycloheximide. The blastocyst formation rate of oocytes treated with 10 microg/ml cycloheximide for 5 h was not significantly different from that of oocytes treated with 40 microg/ml cycloheximide for 2 h. These treatments did not affect the activation status of oocytes compared with controls that were not treated with cycloheximide. The results of the present study showed that cycloheximide improves the parthenogenetic development of pig oocytes activated by ultrasound stimulation.
Authors:
Hironori Mori; Yamato Mizobe; Shin Inoue; Akari Uenohara; Mitsuru Takeda; Mitsutoshi Yoshida; Kazuchika Miyoshi
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-18
Journal Detail:
Title:  The Journal of reproduction and development     Volume:  54     ISSN:  0916-8818     ISO Abbreviation:  J. Reprod. Dev.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-11-06     Completed Date:  2009-02-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9438792     Medline TA:  J Reprod Dev     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  364-9     Citation Subset:  IM    
Affiliation:
Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University, Kagoshima, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Cycloheximide / pharmacology*
Dose-Response Relationship, Drug
Embryonic Development / drug effects,  radiation effects
Female
Oocytes / drug effects*,  radiation effects,  ultrasonography
Oogenesis / drug effects,  radiation effects
Parthenogenesis / drug effects*,  physiology,  radiation effects
Swine / physiology*
Ultrasonics*
Chemical
Reg. No./Substance:
66-81-9/Cycloheximide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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