Document Detail


Effects of cyclic dynamic tensile strain on previously compressed inner annulus fibrosus and nucleus pulposus cells of human intervertebral disc-an in vitro study.
MedLine Citation:
PMID:  19810104     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Our objective was to investigate whether dynamic tensile strain on previously compressed human intervertebral disc (IVD) cells can restore the biosynthetic effects of collagen and glycosaminoglycan. Inner annulus fibrosus (AF) and nucleus pulposus (NP) tissues of adolescent idiopathic scoliosis cases undergoing thoracoscopic discectomy and fusion were cultured on compressive plates. Compressive stress was applied using 0.4 MPa at 1 Hz, for 2 h twice a day for 7 days, to the inner AF and NP tissues, followed by equibiaxial cyclic tensile strain to deform the released cells onto the plate's flexible bottom. With 10% elongation at a rate of 1 Hz, for 2 h twice a day for 7 days, a significant increase in the level of collagen and glycosaminoglycan of the previously compressed inner AF, as well as the level of glycosaminoglycan of the previously compressed NP cells were found. The DNA content and number of endoplasmic reticulum under transmission electron micrograph of the previously compressed inner AF and NP cell were also significantly increased. The results suggested that equibiaxial cyclic tensile strain at a rate of 1 Hz with 10% tensile strain was capable of increasing collagen and glycosaminoglycan synthesis of previously compressed inner AF cells, and glycosaminoglycan synthesis of previously compressed NP cells.
Authors:
Hwan Tak Hee; Jituan Zhang; Hee Kit Wong
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of orthopaedic research : official publication of the Orthopaedic Research Society     Volume:  28     ISSN:  1554-527X     ISO Abbreviation:  J. Orthop. Res.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-02-22     Completed Date:  2010-03-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8404726     Medline TA:  J Orthop Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  503-9     Citation Subset:  IM    
Affiliation:
Department of Orthopedic Surgery, National University Hospital, Yong Loo Lin School of Medicine, National University of Singapore, 5 Lower Kent Ridge Road, Singapore 119074, Singapore. doshht@nus.edu.sg
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MeSH Terms
Descriptor/Qualifier:
Cell Proliferation
Cells, Cultured
Collagen / biosynthesis*
DNA / biosynthesis
Endoplasmic Reticulum / physiology,  ultrastructure
Glycosaminoglycans / biosynthesis*
Humans
Intervertebral Disk / cytology,  metabolism*
Mechanotransduction, Cellular / physiology*
Stress, Mechanical
Tensile Strength / physiology
Chemical
Reg. No./Substance:
0/Glycosaminoglycans; 9007-34-5/Collagen; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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