Document Detail

Effects of the cryopreservation procedures on recovered rice cell populations.
MedLine Citation:
PMID:  11912503     Owner:  NLM     Status:  MEDLINE    
Cryogenic storage of plant cells allows the long-term maintenance of valuable genotypes. Cryopreservation of calli and cell suspensions is often performed using cryoprotectants and slow cooling rates. Rice calli (Oryza sativa L.) were cryopreserved by this procedure as well as by direct immersion in liquid nitrogen without cryoprotection. Subsequently, the characteristics of the recovered cells as well as the effects of putative cryoselection were investigated by microscopic observations and flow cytometric analyses. For this purpose, protoplasts were prepared from calli that had been cryopreserved by direct plunging into liquid nitrogen and from their unfrozen controls. Results show that direct immersion in liquid nitrogen of calli pre-treated with abscisic acid is a fast and highly efficient freezing procedure that maintains the main characteristics of the cell populations and appears to increase their metabolic activity
O Moukadiri; J E O'Connor; M J Cornejo
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cryo letters     Volume:  23     ISSN:  0143-2044     ISO Abbreviation:  Cryo Letters     Publication Date:    2002 Jan-Feb
Date Detail:
Created Date:  2002-03-25     Completed Date:  2002-05-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9891832     Medline TA:  Cryo Letters     Country:  England    
Other Details:
Languages:  eng     Pagination:  11-20     Citation Subset:  IM    
Departamento de Biología Vegetal, Universidad de Valencia, Fac. Biología, Dr. Moliner, 50. 46100 Burjassot, Valencia, Spain.
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MeSH Terms
Abscisic Acid / pharmacology
Cell Division / physiology
Cell Survival / drug effects,  physiology
Cryopreservation / methods*
Cryoprotective Agents / pharmacology
Flow Cytometry / methods
Oryza sativa / cytology*,  drug effects
Plant Growth Regulators / pharmacology
Protoplasts / drug effects,  physiology*
Recovery of Function
Reg. No./Substance:
0/Cryoprotective Agents; 0/Plant Growth Regulators; 21293-29-8/Abscisic Acid; 7727-37-9/Nitrogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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