| Effects of the cryopreservation procedures on recovered rice cell populations. | |
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MedLine Citation:
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PMID: 11912503 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cryogenic storage of plant cells allows the long-term maintenance of valuable genotypes. Cryopreservation of calli and cell suspensions is often performed using cryoprotectants and slow cooling rates. Rice calli (Oryza sativa L.) were cryopreserved by this procedure as well as by direct immersion in liquid nitrogen without cryoprotection. Subsequently, the characteristics of the recovered cells as well as the effects of putative cryoselection were investigated by microscopic observations and flow cytometric analyses. For this purpose, protoplasts were prepared from calli that had been cryopreserved by direct plunging into liquid nitrogen and from their unfrozen controls. Results show that direct immersion in liquid nitrogen of calli pre-treated with abscisic acid is a fast and highly efficient freezing procedure that maintains the main characteristics of the cell populations and appears to increase their metabolic activity |
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Authors:
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O Moukadiri; J E O'Connor; M J Cornejo |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cryo letters Volume: 23 ISSN: 0143-2044 ISO Abbreviation: Cryo Letters Publication Date: 2002 Jan-Feb |
Date Detail:
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Created Date: 2002-03-25 Completed Date: 2002-05-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9891832 Medline TA: Cryo Letters Country: England |
Other Details:
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Languages: eng Pagination: 11-20 Citation Subset: IM |
Affiliation:
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Departamento de Biología Vegetal, Universidad de Valencia, Fac. Biología, Dr. Moliner, 50. 46100 Burjassot, Valencia, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Abscisic Acid
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pharmacology Cell Division / physiology Cell Survival / drug effects, physiology Cryopreservation / methods* Cryoprotective Agents / pharmacology Flow Cytometry / methods Nitrogen Oryza sativa / cytology*, drug effects Plant Growth Regulators / pharmacology Protoplasts / drug effects, physiology* Recovery of Function |
| Chemical | |
Reg. No./Substance:
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0/Cryoprotective Agents; 0/Plant Growth Regulators; 21293-29-8/Abscisic Acid; 7727-37-9/Nitrogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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