Document Detail


Effects of cryopreservation and/or decellularization on extracellular matrix of porcine valves.
MedLine Citation:
PMID:  22086591     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The objective of this study was to evaluate the morphology of decelluarized and/or cryopreserved porcine pulmonary valves, to determine a solution capable of completely remove the cells without damaging the extracellular matrix. Porcine pulmonary valves were incubated for 24 hs in sodium deoxicholate 1% or sodium dodecyl sulfate 0.1 and 0.3%, with or without associated cryopreservation. Evaluation was done with optical microscopy (Hematoxilin-Eosin, Acetic Orcein and Gomori) and with morphometric analysis. The effectiviness of the solutions was variable, but the best results were obtained with the sodium dodecyl sulfate solution 0.1%.
Authors:
Luciana Cristina Ferretti de Nazareno Wollmann; Carlos A H Laurindo; Francisco Diniz Affonso da Costa; Andréa Novais Moreno
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Revista brasileira de cirurgia cardiovascular : orgao oficial da Sociedade Brasileira de Cirurgia Cardiovascular     Volume:  26     ISSN:  1678-9741     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-11-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9104279     Medline TA:  Rev Bras Cir Cardiovasc     Country:  -    
Other Details:
Languages:  ENG; POR     Pagination:  490-496     Citation Subset:  -    
Vernacular Title:
Efeito da criopreservação e/ou da descelularização na matriz extracelular de condutos valvados porcinos.
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