Document Detail


Effects of crude oil on phospholipid fatty acid compositions of marine hydrocarbon degraders: estimation of the bacterial membrane fluidity.
MedLine Citation:
PMID:  15589239     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we investigated, in vitro, the effects of petroleum hydrocarbons on the phospholipid ester-linked fatty acid composition of Corynebacterium sp. Strain 8. The usual ratio of monounsaturated fatty acids E/Z (or trans/cis) was calculated. This ratio led to unexpected results because we found similar values for growths on either a hydrophobic substrate (crude oil) or a soluble carbon source (rich medium). The use of such an indicator seemed limited for monitoring an environmental stress, so we proposed an index based on the homeoviscous adaptation theory. A membrane viscosity index was defined and applied to Corynebacterium sp. Strain 8 (in vitro growth) and to a sedimentary community (in situ experiment). The results allowed us to estimate the membrane fluidity of both an isolated strain and a bacterial community in accordance with the medium hydrophobicity.
Authors:
N Mazzella; A D Syakti; J Molinet; M Gilewicz; P Doumenq; J Artaud; J-C Bertrand
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Environmental research     Volume:  97     ISSN:  0013-9351     ISO Abbreviation:  Environ. Res.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2004-12-13     Completed Date:  2005-02-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0147621     Medline TA:  Environ Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  300-11     Citation Subset:  IM    
Affiliation:
Laboratoire de Chimie Analytique de l'Environnement, Université d'Aix Marseille, UMR 6171, IFR PMSE 112, Europôle de l'Arbois, BP 80, 13545 Aix-en-Provence Cedex 4, France.
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MeSH Terms
Descriptor/Qualifier:
Biodegradation, Environmental
Corynebacterium / drug effects*,  metabolism
Gas Chromatography-Mass Spectrometry
Humans
Membrane Fluidity
Petroleum / toxicity*
Phospholipids / metabolism*
Chemical
Reg. No./Substance:
0/Petroleum; 0/Phospholipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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