| Effects of cotransfection of antisense-EGFR and wild-type PTEN cDNA on human glioblastoma cells. | |
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MedLine Citation:
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PMID: 16771172 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The main molecular genetic changes identified in glioblastomas are overexpression/amplification of the epidermal growth factor receptor (EGFR) gene and mutation/ deletion of the tumor suppressor PTEN gene. These two genetic changes both play important roles in glial tumorigenesis and progression. In this study, we demonstrated that wild-type PTEN transfection inhibited the growth and transforming ability of U87MG cells by 69.3% and 73.5%, respectively. On the other hand, antisense-EGFR transfection inhibited the growth and transforming phenotype of these cells by 50.3% and 46.8%, respectively. However, cotransfection of U87MG cells with wild-type PTEN and antisense EGFR constructs could inhibit the cellular growth by 91.7%. The transforming phenotype of these cells was completely inhibited. In addition, these cotransfected cells showed a differentiated form and expressed much lower telomerase activity than cells transfected with wild-type PTEN or antisense-EGFR alone. In summary, these results suggest that cotransfection is a better approach to suppress glioma cell growth than wild-type PTEN transfer or antisense-EGFR transfection alone. This approach may prove useful as an adjunct therapy in the treatment of glioblastomas. |
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Authors:
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Xin-Xia Tian; Yun-Gang Zhang; Juan Du; Wei-Gang Fang; Ho-Keung Ng; Jie Zheng |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neuropathology : official journal of the Japanese Society of Neuropathology Volume: 26 ISSN: 0919-6544 ISO Abbreviation: Neuropathology Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-06-14 Completed Date: 2006-08-03 Revised Date: 2008-03-10 |
Medline Journal Info:
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Nlm Unique ID: 9606526 Medline TA: Neuropathology Country: Australia |
Other Details:
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Languages: eng Pagination: 178-87 Citation Subset: IM |
Affiliation:
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Department of Pathology, Peking University Health Science Center, Beijing 100083, China. tianxinxia@yahoo.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Blotting, Western Brain Neoplasms / genetics* Cell Line, Tumor Cell Proliferation Cell Transformation, Neoplastic / genetics, metabolism DNA, Antisense DNA, Complementary Epidermal Growth Factor / genetics*, metabolism Glial Fibrillary Acidic Protein / metabolism Glioblastoma / genetics* Humans Immunohistochemistry PTEN Phosphohydrolase / genetics*, metabolism Reverse Transcriptase Polymerase Chain Reaction Telomerase / metabolism Transfection* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Antisense; 0/DNA, Complementary; 0/Glial Fibrillary Acidic Protein; 62229-50-9/Epidermal Growth Factor; EC 2.7.7.49/Telomerase; EC 3.1.3.67/PTEN Phosphohydrolase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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