Document Detail

Effects of cotransfection of antisense-EGFR and wild-type PTEN cDNA on human glioblastoma cells.
MedLine Citation:
PMID:  16771172     Owner:  NLM     Status:  MEDLINE    
The main molecular genetic changes identified in glioblastomas are overexpression/amplification of the epidermal growth factor receptor (EGFR) gene and mutation/ deletion of the tumor suppressor PTEN gene. These two genetic changes both play important roles in glial tumorigenesis and progression. In this study, we demonstrated that wild-type PTEN transfection inhibited the growth and transforming ability of U87MG cells by 69.3% and 73.5%, respectively. On the other hand, antisense-EGFR transfection inhibited the growth and transforming phenotype of these cells by 50.3% and 46.8%, respectively. However, cotransfection of U87MG cells with wild-type PTEN and antisense EGFR constructs could inhibit the cellular growth by 91.7%. The transforming phenotype of these cells was completely inhibited. In addition, these cotransfected cells showed a differentiated form and expressed much lower telomerase activity than cells transfected with wild-type PTEN or antisense-EGFR alone. In summary, these results suggest that cotransfection is a better approach to suppress glioma cell growth than wild-type PTEN transfer or antisense-EGFR transfection alone. This approach may prove useful as an adjunct therapy in the treatment of glioblastomas.
Xin-Xia Tian; Yun-Gang Zhang; Juan Du; Wei-Gang Fang; Ho-Keung Ng; Jie Zheng
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuropathology : official journal of the Japanese Society of Neuropathology     Volume:  26     ISSN:  0919-6544     ISO Abbreviation:  Neuropathology     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-06-14     Completed Date:  2006-08-03     Revised Date:  2008-03-10    
Medline Journal Info:
Nlm Unique ID:  9606526     Medline TA:  Neuropathology     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  178-87     Citation Subset:  IM    
Department of Pathology, Peking University Health Science Center, Beijing 100083, China.
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MeSH Terms
Blotting, Western
Brain Neoplasms / genetics*
Cell Line, Tumor
Cell Proliferation
Cell Transformation, Neoplastic / genetics,  metabolism
DNA, Antisense
DNA, Complementary
Epidermal Growth Factor / genetics*,  metabolism
Glial Fibrillary Acidic Protein / metabolism
Glioblastoma / genetics*
PTEN Phosphohydrolase / genetics*,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Telomerase / metabolism
Reg. No./Substance:
0/DNA, Antisense; 0/DNA, Complementary; 0/Glial Fibrillary Acidic Protein; 62229-50-9/Epidermal Growth Factor; EC; EC Phosphohydrolase

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