Document Detail


Effects of corticosterone deficiency and its replacement on Leydig cell steroidogenesis.
MedLine Citation:
PMID:  18335507     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Clinical and experimental studies have shown the adverse effects of glucocorticoid deficiency/metyrapone treatment on testicular Leydig cell testosterone production. However, molecular mechanisms that underlie the effects of glucocorticoid deficiency on Leydig cell steroidogenesis are not yet determined. Therefore, the present study was designed to assess the mechanism of this phenomenon. Following metyrapone-induced corticosterone deficiency, serum testosterone, and Leydig cell 14C-glucose oxidation were decreased. StAR mRNA and protein levels were significantly increased in Leydig cells of corticosterone-deficient animals. mRNA levels and the specific activities of P(450)scc and 17beta-HSD were decreased by corticosterone deficiency, whereas the activity and mRNA of 3beta-HSD were increased. Simultaneous administration of corticosterone prevented its deficiency-induced changes in Leydig cells. Our results show that metyrapone-induced corticosterone deficiency impairs Leydig cell testosterone production by decreasing the activities of steroidogenic enzymes and their mRNA expression and glucose oxidation.
Authors:
C Parthasarathy; K Balasubramanian
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  104     ISSN:  1097-4644     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-28     Completed Date:  2008-10-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1671-83     Citation Subset:  IM    
Affiliation:
Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600113, Tamil Nadu, India.
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MeSH Terms
Descriptor/Qualifier:
17-Hydroxysteroid Dehydrogenases / genetics,  metabolism
3-Hydroxysteroid Dehydrogenases / genetics,  metabolism
Animals
Blotting, Western
Carbon Radioisotopes
Cholesterol Side-Chain Cleavage Enzyme / genetics,  metabolism
Corticosterone / blood,  deficiency*,  therapeutic use
Gene Expression Regulation / drug effects
Glucose / metabolism
Hormone Replacement Therapy*
Leydig Cells / drug effects,  enzymology,  metabolism*
Male
Metyrapone / pharmacology
Oxidation-Reduction / drug effects
Phosphoproteins / genetics,  metabolism
RNA, Messenger / genetics,  metabolism
Rats
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Testosterone / biosynthesis*,  blood
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Phosphoproteins; 0/RNA, Messenger; 0/steroidogenic acute regulatory protein; 50-22-6/Corticosterone; 50-99-7/Glucose; 54-36-4/Metyrapone; 58-22-0/Testosterone; EC 1.1.-/17-Hydroxysteroid Dehydrogenases; EC 1.1.-/3-Hydroxysteroid Dehydrogenases; EC 1.14.15.6/Cholesterol Side-Chain Cleavage Enzyme

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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