| Effects of corticosterone deficiency and its replacement on Leydig cell steroidogenesis. | |
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MedLine Citation:
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PMID: 18335507 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Clinical and experimental studies have shown the adverse effects of glucocorticoid deficiency/metyrapone treatment on testicular Leydig cell testosterone production. However, molecular mechanisms that underlie the effects of glucocorticoid deficiency on Leydig cell steroidogenesis are not yet determined. Therefore, the present study was designed to assess the mechanism of this phenomenon. Following metyrapone-induced corticosterone deficiency, serum testosterone, and Leydig cell 14C-glucose oxidation were decreased. StAR mRNA and protein levels were significantly increased in Leydig cells of corticosterone-deficient animals. mRNA levels and the specific activities of P(450)scc and 17beta-HSD were decreased by corticosterone deficiency, whereas the activity and mRNA of 3beta-HSD were increased. Simultaneous administration of corticosterone prevented its deficiency-induced changes in Leydig cells. Our results show that metyrapone-induced corticosterone deficiency impairs Leydig cell testosterone production by decreasing the activities of steroidogenic enzymes and their mRNA expression and glucose oxidation. |
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Authors:
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C Parthasarathy; K Balasubramanian |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: 104 ISSN: 1097-4644 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-07-28 Completed Date: 2008-10-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 1671-83 Citation Subset: IM |
Affiliation:
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Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600113, Tamil Nadu, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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17-Hydroxysteroid Dehydrogenases
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genetics,
metabolism 3-Hydroxysteroid Dehydrogenases / genetics, metabolism Animals Blotting, Western Carbon Radioisotopes Cholesterol Side-Chain Cleavage Enzyme / genetics, metabolism Corticosterone / blood, deficiency*, therapeutic use Gene Expression Regulation / drug effects Glucose / metabolism Hormone Replacement Therapy* Leydig Cells / drug effects, enzymology, metabolism* Male Metyrapone / pharmacology Oxidation-Reduction / drug effects Phosphoproteins / genetics, metabolism RNA, Messenger / genetics, metabolism Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction Testosterone / biosynthesis*, blood |
| Chemical | |
Reg. No./Substance:
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0/Carbon Radioisotopes; 0/Phosphoproteins; 0/RNA, Messenger; 0/steroidogenic acute regulatory protein; 50-22-6/Corticosterone; 50-99-7/Glucose; 54-36-4/Metyrapone; 58-22-0/Testosterone; EC 1.1.-/17-Hydroxysteroid Dehydrogenases; EC 1.1.-/3-Hydroxysteroid Dehydrogenases; EC 1.14.15.6/Cholesterol Side-Chain Cleavage Enzyme |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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