Document Detail


EFFECTS OF CORN STEEP LIQUOR AND THIAMINE ON L-GLUTAMIC ACID FERMENTATION OF HYDROCARBONS.IV. UTILIZATION OF HYDROCARBONS BY MICROORGANISMS.
MedLine Citation:
PMID:  14264843     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of nutrients of natural source, such as corn steep liquor, peptone, and yeast extract, on the fermentative production of L-glutamic acid from hydrocarbons by a Corynebacterium was studied. Corn steep liquor and meat extract were found to be remarkably stimulatory to L-glutamic acid production; about 5 g per liter of L-glutamic acid were accumulated in a culture broth containing 3% n-paraffins, 0.01% corn steep liquor, and mineral salts. Among nutritional factors contained in corn steep liquor, biotin had very little effect on the accumulation of L-glutamic acid, but thiamine was highly stimulatory to L-glutamic acid production. The optimal concentration of thiamine for L-glutamic acid production was 3 to 5 mug per liter, and for cell growth, 50 mug per liter. L-Glutamic acid was accumulated in negligible quantity when the amount of thiamine in the culture broth was sufficient to support abundant growth of bacterial cells.
Authors:
J TAKAHASHI; K KOBAYASHI; Y IMADA; K YAMADA
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied microbiology     Volume:  13     ISSN:  0003-6919     ISO Abbreviation:  Appl Microbiol     Publication Date:  1965 Jan 
Date Detail:
Created Date:  1965-06-01     Completed Date:  1996-12-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605802     Medline TA:  Appl Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1-4     Citation Subset:  OM    
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MeSH Terms
Descriptor/Qualifier:
Biotin*
Corynebacterium*
Culture Media*
Fermentation*
Glutamates*
Hydrocarbons*
Metabolism*
Research*
Thiamine*
Chemical
Reg. No./Substance:
0/Culture Media; 0/Glutamates; 0/Hydrocarbons; 58-85-5/Biotin; 59-43-8/Thiamine
Comments/Corrections

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