Document Detail


Effects of conjugated linoleic acid on liver composition and fatty acid oxidation are isomer-dependent in hamster.
MedLine Citation:
PMID:  15811773     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The present work was designed to study the effects of the two main isomers of conjugated linoleic acid (CLA), cis-9,trans-11 and trans-10,cis-12, on liver composition and hepatic fatty acid oxidation in hamsters. METHODS: Animals were divided into three groups that were fed atherogenic diets supplemented with 0.5% linoleic acid, cis-9,trans-11 CLA, or trans-10,cis-12 CLA for 6 wk. Liver lipids, protein, water and DNA contents, and histologic structure were analyzed. Hepatic carnitine palmitoyltransferase-I and acyl coenzyme A oxidase activities were assessed. Triacylglycerol concentration, and aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltransferase, and alkaline phosphatase activities were evaluated in serum. CLA isomer contents were analyzed by gas chromatography in hepatic triacylglycerols. Peroxisome proliferator-activated receptor-alpha mRNA was determined by reverse transcriptase polymerase chain reaction. RESULTS: Trans-10,cis-12 CLA led to significantly greater weight, lower levels of triacylglycerol, cholesterol, and phospholipid, and larger total cell number in liver. Carnitine palmitoyltransferase-I and acyl coenzyme A oxidase activities were significantly increased by this isomer. No changes were induced by cis-9,trans-11 CLA. Trans-10,cis-12 CLA was recovered in significantly lower proportions than cis-9,trans-11 in liver triacylglycerols. Histopathologic analysis showed no abnormalities. No significant differences in serum aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltransferase, and alkaline phosphatase activities or in hepatic mRNA peroxisome proliferator-activated receptor-alpha expression were found among the three experimental groups. CONCLUSIONS: These results suggest that the addition of 0.5% of these CLA isomers to the diet do not induce toxic effects in liver after 6 wk of feeding. Intake of trans-10,cis-12 isomer but not of cis-9,trans-11 CLA increases liver fatty acid oxidation. This effect leads to decreased hepatic and serum triacylglycerols.
Authors:
M Teresa Macarulla; Alfredo Fernández-Quintela; Amaia Zabala; Virginia Navarro; Enrique Echevarría; Itziar Churruca; Víctor M Rodríguez; María P Portillo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nutrition (Burbank, Los Angeles County, Calif.)     Volume:  21     ISSN:  0899-9007     ISO Abbreviation:  Nutrition     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-04-06     Completed Date:  2005-09-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8802712     Medline TA:  Nutrition     Country:  United States    
Other Details:
Languages:  eng     Pagination:  512-9     Citation Subset:  IM    
Affiliation:
Department of Nutrition and Food Science, Faculty of Pharmacy, University of País Vasco, Vitoria, Spain.
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MeSH Terms
Descriptor/Qualifier:
Acyl-CoA Oxidase / drug effects,  metabolism
Alkaline Phosphatase / blood
Animals
Body Weight / drug effects
Carnitine O-Palmitoyltransferase / drug effects,  metabolism
Chromatography, Gas / methods
Cricetinae
DNA / drug effects,  metabolism
Fatty Acids / metabolism*
Isomerism
Linoleic Acids, Conjugated / pharmacology*
Lipid Metabolism
Liver / drug effects*,  enzymology,  metabolism*
Male
Organ Size / drug effects
Oxidation-Reduction / drug effects
Proteins / metabolism
Reverse Transcriptase Polymerase Chain Reaction / methods
Time Factors
Transferases / blood
Triglycerides / blood
Water / metabolism
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Linoleic Acids, Conjugated; 0/Proteins; 0/Triglycerides; 7732-18-5/Water; 9007-49-2/DNA; EC 1.3.3.6/Acyl-CoA Oxidase; EC 2.-/Transferases; EC 2.3.1.21/Carnitine O-Palmitoyltransferase; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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