| Effects of conjugated linoleic acid on liver composition and fatty acid oxidation are isomer-dependent in hamster. | |
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MedLine Citation:
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PMID: 15811773 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The present work was designed to study the effects of the two main isomers of conjugated linoleic acid (CLA), cis-9,trans-11 and trans-10,cis-12, on liver composition and hepatic fatty acid oxidation in hamsters. METHODS: Animals were divided into three groups that were fed atherogenic diets supplemented with 0.5% linoleic acid, cis-9,trans-11 CLA, or trans-10,cis-12 CLA for 6 wk. Liver lipids, protein, water and DNA contents, and histologic structure were analyzed. Hepatic carnitine palmitoyltransferase-I and acyl coenzyme A oxidase activities were assessed. Triacylglycerol concentration, and aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltransferase, and alkaline phosphatase activities were evaluated in serum. CLA isomer contents were analyzed by gas chromatography in hepatic triacylglycerols. Peroxisome proliferator-activated receptor-alpha mRNA was determined by reverse transcriptase polymerase chain reaction. RESULTS: Trans-10,cis-12 CLA led to significantly greater weight, lower levels of triacylglycerol, cholesterol, and phospholipid, and larger total cell number in liver. Carnitine palmitoyltransferase-I and acyl coenzyme A oxidase activities were significantly increased by this isomer. No changes were induced by cis-9,trans-11 CLA. Trans-10,cis-12 CLA was recovered in significantly lower proportions than cis-9,trans-11 in liver triacylglycerols. Histopathologic analysis showed no abnormalities. No significant differences in serum aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltransferase, and alkaline phosphatase activities or in hepatic mRNA peroxisome proliferator-activated receptor-alpha expression were found among the three experimental groups. CONCLUSIONS: These results suggest that the addition of 0.5% of these CLA isomers to the diet do not induce toxic effects in liver after 6 wk of feeding. Intake of trans-10,cis-12 isomer but not of cis-9,trans-11 CLA increases liver fatty acid oxidation. This effect leads to decreased hepatic and serum triacylglycerols. |
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Authors:
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M Teresa Macarulla; Alfredo Fernández-Quintela; Amaia Zabala; Virginia Navarro; Enrique Echevarría; Itziar Churruca; Víctor M Rodríguez; María P Portillo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Nutrition (Burbank, Los Angeles County, Calif.) Volume: 21 ISSN: 0899-9007 ISO Abbreviation: Nutrition Publication Date: 2005 Apr |
Date Detail:
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Created Date: 2005-04-06 Completed Date: 2005-09-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8802712 Medline TA: Nutrition Country: United States |
Other Details:
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Languages: eng Pagination: 512-9 Citation Subset: IM |
Affiliation:
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Department of Nutrition and Food Science, Faculty of Pharmacy, University of País Vasco, Vitoria, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acyl-CoA Oxidase
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drug effects,
metabolism Alkaline Phosphatase / blood Animals Body Weight / drug effects Carnitine O-Palmitoyltransferase / drug effects, metabolism Chromatography, Gas / methods Cricetinae DNA / drug effects, metabolism Fatty Acids / metabolism* Isomerism Linoleic Acids, Conjugated / pharmacology* Lipid Metabolism Liver / drug effects*, enzymology, metabolism* Male Organ Size / drug effects Oxidation-Reduction / drug effects Proteins / metabolism Reverse Transcriptase Polymerase Chain Reaction / methods Time Factors Transferases / blood Triglycerides / blood Water / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Linoleic Acids, Conjugated; 0/Proteins; 0/Triglycerides; 7732-18-5/Water; 9007-49-2/DNA; EC 1.3.3.6/Acyl-CoA Oxidase; EC 2.-/Transferases; EC 2.3.1.21/Carnitine O-Palmitoyltransferase; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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