| Effects of conjugated linoleic acid isomers on lipid metabolism and gluconeogenesis in monolayer cultures of bovine hepatocytes. | |
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MedLine Citation:
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PMID: 14765812 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective was to determine the effects of linoleic acid and different isomers of conjugated linoleic acid (CLA) at different concentrations on hepatic lipid and glucose metabolism in the bovine. Monolayer cultures of hepatocytes obtained from 7- to 10-d-old Holstein bull calves were exposed to treatments from 16 to 64 h after plating. The treatments included 1.0 mM palmitic acid plus either 0.1 or 1.0 mM of cis-9, cis-12 linoleic acid, cis-9, trans-11 CLA, or trans-10, cis-12 CLA. Metabolism of palmitic acid to cellular triacylglycerol (TAG) was decreased when media contained cis-9, trans-11 compared with trans-10, cis-12 CLA. Total cellular TAG content was increased for the CLA isomers compared to cis-9, cis-12 linoleic acid. Both CLA isomers increased palmitic acid incorporation into phospholipids, cholesterol, and media triacylglycerol compared with cis-9, cis-12 linoleic acid at a concentration of 1.0 mM. Increasing the concentration of treatment fatty acids from 0.1 to 1.0 mM decreased oxidation of palmitic acid to acid-soluble products, but no effects of fatty acids were observed. There was no treatment effect on rates of gluconeogenesis from propionic acid. Overall, CLA isomers elicited changes in palmitic acid metabolism to cellular and media triacylglycerol, and cellular phospholipids and cholesterol, but had little or no effect on other measured pathways of lipid metabolism or gluconeogenesis in bovine hepatocytes. |
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Authors:
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D G Mashek; R R Grummer |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of dairy science Volume: 87 ISSN: 0022-0302 ISO Abbreviation: J. Dairy Sci. Publication Date: 2004 Jan |
Date Detail:
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Created Date: 2004-02-09 Completed Date: 2004-04-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985126R Medline TA: J Dairy Sci Country: United States |
Other Details:
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Languages: eng Pagination: 67-72 Citation Subset: IM |
Affiliation:
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Department of Dairy Science, University of Wisconsin, Madison 53706, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle / metabolism* Cells, Cultured Cholesterol / metabolism Gluconeogenesis / drug effects* Hepatocytes / drug effects*, metabolism Linoleic Acids, Conjugated / pharmacology* Lipid Metabolism* Male Oxidation-Reduction Palmitic Acid / metabolism Phospholipids / metabolism Rats Triglycerides / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Linoleic Acids, Conjugated; 0/Phospholipids; 0/Triglycerides; 57-10-3/Palmitic Acid; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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