| Effects of conjugated linoleic acid on myogenic and inflammatory responses in a human primary muscle and tumor coculture model. | |
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MedLine Citation:
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PMID: 19838943 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The antiproliferative and anti-inflammatory properties of conjugated linoleic acid (CLA) make it a potentially novel treatment in chronic inflammatory muscle wasting disease, particularly cancer cachexia. Human primary muscle cells were grown in coculture with MIA PaCa-2 pancreatic tumor cells and exposed to varying concentrations of c9,t11 and t10,c12 CLA. Expression of myogenic (Myf5, MyoD, myogenin, and myostatin) and inflammatory genes (CCL-2, COX-2, IL-8, and TNF-alpha) were measured by real-time PCR. The t10,c12 CLA isomer, but not the c9,t11 isomer, significantly decreased MIA PaCa-2 proliferation by between 15% and 19%. There was a marked decrease in muscle MyoD and myogenin expression (78% and 62%, respectively), but no change in either Myf5 or myostatin, in myotubes grown in coculture with MIA PaCa-2 cells. CLA had limited influence on these responses. A similar pattern of myogenic gene expression changes was observed in myotubes treated with TNF-alpha alone. Several-fold significant increases in CCL-2, COX-2, IL-8, and TNF-alpha expression in myotubes were observed with MIA PaCa-2 coculture. The c9,t11 CLA isomer significantly decreased basal expression of TNF-alpha in myotubes and could ameliorate its tumor-induced rise. The study provides insight into the anti-inflammatory and antiproliferative actions of CLA and its application as a therapeutic agent in inflammatory disease states. |
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Authors:
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Amy E Larsen; Timothy C Crowe |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Nutrition and cancer Volume: 61 ISSN: 1532-7914 ISO Abbreviation: Nutr Cancer Publication Date: 2009 |
Date Detail:
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Created Date: 2009-10-19 Completed Date: 2010-01-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7905040 Medline TA: Nutr Cancer Country: United States |
Other Details:
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Languages: eng Pagination: 687-95 Citation Subset: IM |
Affiliation:
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School of Exercise and Nutrition Sciences, Deakin University, Burwood, Victoria, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anti-Inflammatory Agents / pharmacology* Antineoplastic Agents / pharmacology* Cell Line, Tumor Cell Proliferation Cells, Cultured Coculture Techniques Cyclooxygenase 2 / genetics, metabolism Cytokines / genetics, metabolism Gene Expression Regulation Humans Inflammation / drug therapy, genetics, metabolism, physiopathology* Isomerism Linoleic Acids, Conjugated / pharmacology* Male Mice Muscle Cells Muscle Development / drug effects*, genetics Muscle Proteins / genetics, metabolism RNA, Messenger / metabolism Tumor Necrosis Factor-alpha / genetics, metabolism, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Anti-Inflammatory Agents; 0/Antineoplastic Agents; 0/Cytokines; 0/Linoleic Acids, Conjugated; 0/Muscle Proteins; 0/RNA, Messenger; 0/Tumor Necrosis Factor-alpha; EC 1.14.99.1/Cyclooxygenase 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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