Document Detail

Effects of conjugated linoleic acid on myogenic and inflammatory responses in a human primary muscle and tumor coculture model.
MedLine Citation:
PMID:  19838943     Owner:  NLM     Status:  MEDLINE    
The antiproliferative and anti-inflammatory properties of conjugated linoleic acid (CLA) make it a potentially novel treatment in chronic inflammatory muscle wasting disease, particularly cancer cachexia. Human primary muscle cells were grown in coculture with MIA PaCa-2 pancreatic tumor cells and exposed to varying concentrations of c9,t11 and t10,c12 CLA. Expression of myogenic (Myf5, MyoD, myogenin, and myostatin) and inflammatory genes (CCL-2, COX-2, IL-8, and TNF-alpha) were measured by real-time PCR. The t10,c12 CLA isomer, but not the c9,t11 isomer, significantly decreased MIA PaCa-2 proliferation by between 15% and 19%. There was a marked decrease in muscle MyoD and myogenin expression (78% and 62%, respectively), but no change in either Myf5 or myostatin, in myotubes grown in coculture with MIA PaCa-2 cells. CLA had limited influence on these responses. A similar pattern of myogenic gene expression changes was observed in myotubes treated with TNF-alpha alone. Several-fold significant increases in CCL-2, COX-2, IL-8, and TNF-alpha expression in myotubes were observed with MIA PaCa-2 coculture. The c9,t11 CLA isomer significantly decreased basal expression of TNF-alpha in myotubes and could ameliorate its tumor-induced rise. The study provides insight into the anti-inflammatory and antiproliferative actions of CLA and its application as a therapeutic agent in inflammatory disease states.
Amy E Larsen; Timothy C Crowe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nutrition and cancer     Volume:  61     ISSN:  1532-7914     ISO Abbreviation:  Nutr Cancer     Publication Date:  2009  
Date Detail:
Created Date:  2009-10-19     Completed Date:  2010-01-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7905040     Medline TA:  Nutr Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  687-95     Citation Subset:  IM    
School of Exercise and Nutrition Sciences, Deakin University, Burwood, Victoria, Australia.
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MeSH Terms
Anti-Inflammatory Agents / pharmacology*
Antineoplastic Agents / pharmacology*
Cell Line, Tumor
Cell Proliferation
Cells, Cultured
Coculture Techniques
Cyclooxygenase 2 / genetics,  metabolism
Cytokines / genetics,  metabolism
Gene Expression Regulation
Inflammation / drug therapy,  genetics,  metabolism,  physiopathology*
Linoleic Acids, Conjugated / pharmacology*
Muscle Cells
Muscle Development / drug effects*,  genetics
Muscle Proteins / genetics,  metabolism
RNA, Messenger / metabolism
Tumor Necrosis Factor-alpha / genetics,  metabolism,  pharmacology
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/Antineoplastic Agents; 0/Cytokines; 0/Linoleic Acids, Conjugated; 0/Muscle Proteins; 0/RNA, Messenger; 0/Tumor Necrosis Factor-alpha; EC 2

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