Document Detail


Effects of commercial pectolytic and cellulolytic enzyme preparations on the apple cell wall.
MedLine Citation:
PMID:  11559117     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The action of three different commercial enzyme combinations on apple cell wall material has been examined in a model system under conditions of mash and pomace treatment by using an alcohol-insoluble substance prepared from apples. A part of the total dietary fiber, for example, galacturonan (pectin), appeared in the soluble fraction after enzymatic mash treatment. The soluble fraction increased intensely during pomace treatment. Furthermore, enzyme actions caused a change in the water-binding capacity of residues as well as changes in the monosaccharide composition and in the molecular weight distribution of saccharides in filtrates (soluble parts). The extent of decomposition of cell wall material and the increase of soluble oligomeric and/or polymeric dietary fiber components are caused by both the composition (pectinases, cellulases, and hemicellulases) and the activities of the enzyme preparations. The model experiments allow an insight into the reactions occurring during enzyme action on the plant cell wall, for example, during apple juice production using pectolytic and cellulolytic enzyme preparations.
Authors:
G Dongowski; S Sembries
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  49     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-09-17     Completed Date:  2001-10-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4236-42     Citation Subset:  IM    
Affiliation:
Department of Food Science and Preventive Nutrition, German Institute of Human Nutrition Potsdam-Rehbrücke, Arthur-Scheunert-Allee 114-116, D-14558 Bergholz-Rehbrücke, Germany. dongo@www.dife.de
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MeSH Terms
Descriptor/Qualifier:
Cell Wall / enzymology*,  metabolism
Cellulase / metabolism*
Dietary Fiber / analysis
Food Handling / methods
Molecular Weight
Pectins / metabolism
Polygalacturonase / metabolism*
Rosales
Solubility
Chemical
Reg. No./Substance:
0/Pectins; EC 3.2.1.15/Polygalacturonase; EC 3.2.1.4/Cellulase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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