Document Detail

Effects of collagen 1, fibronectin, laminin and hyaluronic acid concentration in multi-component gels on neurite extension.
MedLine Citation:
PMID:  17705994     Owner:  NLM     Status:  MEDLINE    
Recovery after peripheral nerve injury remains a significant challenge. Extracellular matrix proteins and hydrogels of extracellular matrix components have been shown to improve regeneration in peripheral nerve entubulation models, especially over long distances. The chemical properties, ligand identity and density, and mechanical properties of the hydrogel can affect neurite extension. However, the importance of combinatorial effects between different components in co-gels of several extracellular matrix components is unclear. In this study, we investigated neurite extension from explanted dorsal root ganglia cultured within co-gels made from laminin, fibronectin, collagen 1 and hyaluronic acid. Laminin had a strong, dose-dependent effect on both neurite length and outgrowth. Fibronectin was slightly, but generally not significantly, inhibitory to neurite extension. The concentration of collagen 1 and hyaluronic acid did not have significant effects on neurite extension. The combinatorial effects among the four components were additive rather than synergistic. A co-gel made with 1.5 mg/ml collagen 1 and 1.5 mg/ml laminin was optimum in this study, resulting in an average neurite length of 1532 +/- 91 microm versus 976 +/- 32 microm for controls, and an increase in overall volume outgrowth (reflecting neurite length and branching) of 85.9+/-9.3% over controls. This co-gel provides a mechanically stable scaffold with high ligand density and biochemical affinity. The results of this study support the use of co-gels of laminin and collagen 1 for promoting regeneration in peripheral nerve injuries and suggest that interactions among hydrogel components are not significant.
Curt Deister; Samer Aljabari; Christine E Schmidt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomaterials science. Polymer edition     Volume:  18     ISSN:  0920-5063     ISO Abbreviation:  J Biomater Sci Polym Ed     Publication Date:  2007  
Date Detail:
Created Date:  2007-08-20     Completed Date:  2007-11-09     Revised Date:  2008-02-20    
Medline Journal Info:
Nlm Unique ID:  9007393     Medline TA:  J Biomater Sci Polym Ed     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  983-97     Citation Subset:  IM    
Department of Chemical Engineering, The University of Texas at Austin, 1 University Station, MC C0400, Austin, TX 78712, USA.
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MeSH Terms
Collagen Type I / metabolism*
Dose-Response Relationship, Drug
Extracellular Matrix / metabolism
Fibronectins / chemistry,  metabolism*
Ganglia, Spinal / metabolism
Hyaluronic Acid / metabolism*
Hydrogel / chemistry
Hydrogels / metabolism
Laminin / chemistry,  metabolism*
Neurites / metabolism*
Rats, Sprague-Dawley
Rheology / methods
Reg. No./Substance:
0/Collagen Type I; 0/Fibronectins; 0/Hydrogels; 0/Laminin; 0/Ligands; 25852-47-5/Hydrogel; 9004-61-9/Hyaluronic Acid

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