| Effects of clonidine on lidocaine-induced inhibition of axonal transport in cultured mouse dorsal root ganglion neurones. | |
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MedLine Citation:
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PMID: 18791189 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The alpha(2)-adrenoceptor agonist clonidine is used in combination with lidocaine for anaesthesia. Lidocaine inhibits axonal transport and neurite growth, whereas alpha(2)-adrenoceptor agonists have neurotrophic effects. Here we have investigated whether clonidine reduces lidocaine-induced inhibition of axonal transport in cultured mouse dorsal root ganglion neurones. METHODS: Axonal transport of organelles and neurite growth were assessed by video microscopy in cells treated with clonidine and lidocaine for 1 h. Stable responses were achieved within this period. RESULTS: Clonidine (10 and 100 microM) increased and lidocaine (10, 100 microM, and 1 mM) decreased axonal transport. The inhibitory effects of lidocaine were reduced by simultaneous treatment with clonidine. The actions of clonidine were antagonized by the alpha(2)-adrenoceptor antagonist yohimbine. Since clonidine was reported to block N-type channels, we further investigated the role of ion channels in the antagonistic action of clonidine on the lidocaine response. The action of lidocaine on axonal transport was not mimicked by the Na+ channel blocker tetrodotoxin and not blocked by the Na+ channel activator veratridine. The action of lidocaine was not blocked by the L-type Ca2+ channel blocker nifedipine, but was blocked by the N-type channel blocker omega-conotoxin MVIIA. These effects on axonal transport correlated with the effects on neurite growth. CONCLUSIONS: Inhibition of axonal transport induced by lidocaine, which may be mediated by N-type channel activation, can be blocked by clonidine. Clonidine may alleviate the effects of lidocaine on neuronal dysfunction. |
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Authors:
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H Hiruma; K Shimizu; T Takenami; H Sugie; T Kawakami |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-12 |
Journal Detail:
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Title: British journal of anaesthesia Volume: 101 ISSN: 1471-6771 ISO Abbreviation: Br J Anaesth Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-10-15 Completed Date: 2008-11-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372541 Medline TA: Br J Anaesth Country: England |
Other Details:
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Languages: eng Pagination: 659-65 Citation Subset: IM |
Affiliation:
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Department of Physiology, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara 228-8555, Japan. hiruma@med.kitasato-u.ac.jp |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adrenergic alpha-Agonists
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pharmacology* Anesthetics, Local / antagonists & inhibitors, pharmacology Animals Axonal Transport / drug effects* Clonidine / pharmacology* Dose-Response Relationship, Drug Ganglia, Spinal / drug effects*, physiology Lidocaine / antagonists & inhibitors*, pharmacology Male Mice Mice, Inbred C57BL Microscopy, Video Neural Inhibition / drug effects Neurites / drug effects, physiology Organelles / drug effects, physiology |
| Chemical | |
Reg. No./Substance:
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0/Adrenergic alpha-Agonists; 0/Anesthetics, Local; 137-58-6/Lidocaine; 4205-90-7/Clonidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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