| Effects of citric acid on cultured human osteoblastic cells. | |
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MedLine Citation:
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PMID: 20955953 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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We investigated the effects of citric acid (CA) on cultured human osteoblastic (HOB) cells by evaluating cell adhesion, proliferation, and cytotoxicity. (3)H-Thymidine-labeled HOB cells were incubated in culture medium supplemented or not with 4%, 6%, 8%, or 10% CA for 1 minute. After incubation, cell morphology was evaluated by Nomarski interferential light microscopy, cell proliferation was accessed by measurements of (3)H-thymidine associated to the cells, and cell lysis was monitored by measuring the amount of (3)H-thymidine released by cells. We observed that most of the CA-treated cells presented numerous atypical vacuoles, and such cells were also highly polymorphic, exhibiting round-shaped cells. Nonetheless, CA at all concentrations assayed did not yield cytotoxicity as measured by (3)H-containing DNA release, although significant decrease in cell proliferation was observed (P > .05). Furthermore, cells which were treated with CA at the lowest concentration assayed (4%) restored normal proliferation rates 3 days after treatment. |
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Authors:
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Lizandra Ferrari Guimarães; Tatiana Kelly da Silva Fidalgo; Gustavo Conde Menezes; Laura Guimarães Primo; Fernando Costa e Silva-Filho |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics Volume: 110 ISSN: 1528-395X ISO Abbreviation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-19 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9508562 Medline TA: Oral Surg Oral Med Oral Pathol Oral Radiol Endod Country: United States |
Other Details:
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Languages: eng Pagination: 665-9 Citation Subset: D; IM |
Copyright Information:
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Copyright © 2010 Mosby, Inc. All rights reserved. |
Affiliation:
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Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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