Document Detail

Effects of citric acid and EDTA conditioning on exposed root dentin: An immunohistochemical analysis of collagen and proteoglycans.
MedLine Citation:
PMID:  17098210     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Preservation of structural and biochemical properties of the root dentin matrix is crucial to favor healing and regenerative periodontal processes. Aim of this study was to evaluate the biochemical characteristics of collagen and chondroitin sulphate of root dentin surfaces exposed by periodontal disease after acid conditioning by means of an immunohistochemical technique. DESIGN: Human teeth scheduled for extraction due to periodontal reason were submitted to: (A) scaling and root planning; (B) ultrasonic instrumentation; (C) no instrumentation. Teeth were then exposed to: (1) 10% citric acid; (2) 17% EDTA; (3) no etching. A double immunolabeling technique was performed to identify type-I collagen and proteoglycans and analyzed under FEI-SEM. RESULTS: Use of 10% citric acid revealed intense labeling for collagen fibrils and proteoglycans; lower labeling was found after EDTA conditioning. Unetched specimens showed residual smear layer on the dentin surface resulting in no evident surface labeling. CONCLUSIONS: This study supports the hypothesis that manual or ultrasonic instrumentation alone is not able to expose the sound dentin matrix, whereas a subsequent acidic conditioning exposes collagen fibrils and associated proteoglycans. The immunohistochemical technique revealed that despite their acidity, both citric acid and EDTA were able to preserve the structural and biochemical properties of the exposed dentin matrix.
Alessandra Ruggeri; Carlo Prati; Annalisa Mazzoni; Cesare Nucci; Roberto Di Lenarda; Giovanni Mazzotti; Lorenzo Breschi
Publication Detail:
Type:  Journal Article     Date:  2006-11-13
Journal Detail:
Title:  Archives of oral biology     Volume:  52     ISSN:  0003-9969     ISO Abbreviation:  Arch. Oral Biol.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2006-12-12     Completed Date:  2007-11-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1-8     Citation Subset:  D; IM    
Department of SAU & FAL, University of Bologna, Italy.
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MeSH Terms
Acid Etching, Dental
Chelating Agents / pharmacology*
Chondroitin Sulfates / metabolism
Citric Acid / pharmacology*
Collagen / metabolism
Dentin / drug effects*
Edetic Acid / pharmacology*
Image Processing, Computer-Assisted / methods
Immunohistochemistry / methods
Microscopy, Electron, Scanning / methods
Proteoglycans / metabolism
Tooth Root / drug effects*,  ultrastructure
Reg. No./Substance:
0/Chelating Agents; 0/Proteoglycans; 60-00-4/Edetic Acid; 77-92-9/Citric Acid; 9007-28-7/Chondroitin Sulfates; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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