Document Detail


Effects of cigarette smoking on Fas/Fas ligand expression of human lymphocytes.
MedLine Citation:
PMID:  10066346     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cigarette smoking has been shown to affect human immune responses. We have studied Fas/Fas ligand (FasL) expression, which is involved in the cytotoxic activity, immune privilege, and self-tolerance, and other apoptosis-associated molecule expression of peripheral blood lymphocytes (PBL) in healthy subjects with/without cigarette smoking. We found that expression of FasL protein was detected marginally in the fresh PBL and was induced upon mitogen activation in normal individuals without smoking. In contrast, fresh PBL from those with chronic cigarette smoking exhibited enhanced expression of FasL protein without in vitro mitogen stimulation. Moreover, mitogen stimulation failed to augment FasL protein expression of their lymphocytes, suggesting dysregulation of FasL expression of PBL in individuals with cigarette smoking. In contrast, Fas, Bcl-2, and p53 expression were not significantly different between normal individuals with chronic cigarette smoking and those without smoking. In addition, we found that in vitro brief treatment with nicotine induces and/or enhances FasL mRNA and protein expression of lymphocytes from normal donors without smoking. These results suggest that aberrant FasL expression of lymphocytes is, at least in part, involved in the immune impairment in individuals with chronic cigarette smoking.
Authors:
N Suzuki; S Wakisaka; Y Takeba; S Mihara; T Sakane
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular immunology     Volume:  192     ISSN:  0008-8749     ISO Abbreviation:  Cell. Immunol.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-04-20     Completed Date:  1999-04-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1246405     Medline TA:  Cell Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  48-53     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Affiliation:
Departments of Immunology and Medicine, St. Marianna University School of Medicine, Kanagawa, 216-8511, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adult
Antigens, CD95 / biosynthesis*
Cells, Cultured
Fas Ligand Protein
Gene Expression
Humans
Leukocytes, Mononuclear / cytology,  drug effects,  metabolism
Membrane Glycoproteins / biosynthesis*
Middle Aged
Nicotine / pharmacology
Plants, Toxic
Proto-Oncogene Proteins c-bcl-2 / biosynthesis
Smoking / metabolism*
T-Lymphocytes / drug effects,  metabolism*
Tobacco
Tumor Suppressor Protein p53 / biosynthesis
Chemical
Reg. No./Substance:
0/Antigens, CD95; 0/FASLG protein, human; 0/Fas Ligand Protein; 0/Membrane Glycoproteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/Tumor Suppressor Protein p53; 54-11-5/Nicotine

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