Document Detail

Effects of chronic ethanol consumption on the synthesis of polypeptides encoded by the hepatic mitochondrial genome.
MedLine Citation:
PMID:  2169877     Owner:  NLM     Status:  MEDLINE    
Liver mitochondria from rats fed ethanol chronically demonstrate an impaired ability to incorporate [35S]methionine into polypeptide products in vitro. This ethanol-induced effect on mitochondrial translation in vitro could not be attributed to significant differences in the methionine precursor pool sizes of ethanol and control mitochondria or to the acute effects of residual ethanol. The observed reduction of radiolabeled methionine incorporation into mitochondrial gene products of ethanol mitochondria in vitro reflects a decrease in the synthesis of all the mitochondrial gene products. However, the percentage of total radiolabel incorporated into each gene product is unaffected by ethanol, suggesting an ethanol-induced coordinate depression of mitochondrial protein synthesis. Moreover, SDS-PAGE and densitometry of submitochondrial particles from ethanol-fed and control rats demonstrated that the steady-state concentration of each of the mitochondrial gene products is decreased in ethanol-fed rats. This reduction of the steady-state concentration of the mitochondrial gene products may be related to the observed depressions of oxidative phosphorylation activities associated with hepatic mitochondria from ethanol-fed rats.
W B Coleman; C C Cunningham
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1019     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1990 Aug 
Date Detail:
Created Date:  1990-10-31     Completed Date:  1990-10-31     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  142-50     Citation Subset:  IM    
Department of Biochemistry, Wake Forest University Medical Center, Winston-Salem, NC.
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MeSH Terms
Alcoholism / metabolism*
DNA, Mitochondrial / drug effects,  genetics*
Electron Transport Complex IV / biosynthesis,  genetics,  isolation & purification
Ethanol / pharmacology
Gene Expression / drug effects*
Macromolecular Substances
Methionine / metabolism
Mitochondria, Liver / drug effects,  metabolism*
Peptide Biosynthesis
Rats, Inbred Strains
Reference Values
Grant Support
2887//PHS HHS; T32 CA 09422/CA/NCI NIH HHS
Reg. No./Substance:
0/DNA, Mitochondrial; 0/Macromolecular Substances; 63-68-3/Methionine; 64-17-5/Ethanol; EC Transport Complex IV

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