| Effects of chronic central leptin infusion on proopiomelanocortin and neurotensin gene expression in the rat hypothalamus. | |
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MedLine Citation:
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PMID: 18562101 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Leptin signaling in the hypothalamus is critical for normal food intake and body weight regulation. While hyperleptinemia in obese people suggests a state of leptin resistance, the mechanism is not clearly understood. In a rat model of central leptin infusion in which animals develop resistance to the satiety action of leptin, orexigenic peptide producing neuropeptide Y neurons in the hypothalamus develop leptin resistance. However, it is still unknown if increased hypothalamic leptin tone caused by central leptin infusion results in the development of leptin resistance in anorexigenic peptide producing proopiomelanocortin (POMC) and neurotensin (NT) neurons. To this end, male rats were infused chronically with leptin (160 ng/h) or vehicle into the lateral cerebroventricle for 16 days. On day 4 of leptin infusion when food intake was decreased, POMC and NT mRNA levels, as determined by RNAse protection assay, were significantly increased as compared to control. By contrast, on day 16 of leptin infusion, when food intake was mostly normalized, both POMC and NT mRNA levels remained unchanged compared with control. These findings suggest the development of leptin resistance in the POMC and NT neurons following chronic elevation of hypothalamic leptin tone, which may be involved in the development of resistance to the satiety action of leptin following central infusion of this peptide hormone. |
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Authors:
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Abhiram Sahu |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2008-05-28 |
Journal Detail:
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Title: Neuroscience letters Volume: 440 ISSN: 0304-3940 ISO Abbreviation: Neurosci. Lett. Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-06-23 Completed Date: 2008-09-15 Revised Date: 2010-09-22 |
Medline Journal Info:
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Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: Ireland |
Other Details:
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Languages: eng Pagination: 125-9 Citation Subset: IM |
Affiliation:
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Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Magee-Womens Research Institute, 204 Craft Avenue, Room B303, Pittsburgh, PA 15213, USA. asahu@pitt.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Appetite Regulation / drug effects Body Weight / drug effects Eating / drug effects Gene Expression / drug effects* Hypothalamus / drug effects*, metabolism Injections, Intraventricular Leptin / administration & dosage, pharmacology* Male Neurons / drug effects, metabolism Neurotensin / genetics* Nuclease Protection Assays / methods Pro-Opiomelanocortin / genetics* RNA, Messenger / genetics, isolation & purification, metabolism Rats Rats, Sprague-Dawley Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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R01 DK061499-01A1/DK/NIDDK NIH HHS; R01 DK61499/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Leptin; 0/RNA, Messenger; 39379-15-2/Neurotensin; 66796-54-1/Pro-Opiomelanocortin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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