Document Detail

Effects of chilling on sampling of bacteria attached to swine carcasses.
MedLine Citation:
PMID:  11264754     Owner:  NLM     Status:  MEDLINE    
Two microbiological sampling techniques, excision and sponge swabbing, were compared by determining counts of aerobic bacteria, coliforms and injured coliforms from 20 de-haired swine carcasses before and after chilling. Excised jowl skin produced significantly greater counts of the three types of bacteria than sponge swabs. Aerobic bacteria, coliforms and injured coliforms recovered by sponge swabbing carcasses before chilling were 11.6%, 0.9% and 11.0% of excised samples, respectively; the corresponding percentages recovered after chilling were 23.9%, 11.1% and 5.0%. Numbers of all bacteria present on the post-chill carcasses were substantially lower than on the pre-chill carcasses. Excision usually produced more countable plates for coliforms and injured coliforms on chilled carcasses than sponge swabbing and therefore, is more suitable in estimating low numbers of faecal bacteria on chilled carcasses. To explore the possible structural bases for these findings, skin samples were inoculated with 10(2)-10(7) cfu cm(-2) faecal bacteria and examined by scanning electron microscopy. Chilled samples showed bacteria and biofilm embedded in superficial crevices, which underlies a possible reason for the lower recovery of bacterial cells by the sponge swabbing. The study indicates that the differences between sampling techniques may be a result of the chilling process of swine carcasses.
S L Yu; P H Cooke; S I Tu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Letters in applied microbiology     Volume:  32     ISSN:  0266-8254     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-03-26     Completed Date:  2001-05-31     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  205-10     Citation Subset:  IM    
Microbial Biophysics & Biochemistry & Core Technologies, Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Wyndmoor, PA 19038, USA.
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MeSH Terms
Bacteria / growth & development*,  isolation & purification
Cold Temperature
Colony Count, Microbial
Culture Media
Meat / microbiology*
Microscopy, Electron, Scanning
Skin / microbiology*
Reg. No./Substance:
0/Culture Media

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