Document Detail


Effects of chemostimuli on [Ca2+]i responses of rat aortic body type I cells and endogenous local neurons: comparison with carotid body cells.
MedLine Citation:
PMID:  22431340     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mammalian aortic bodies (ABs) are putative peripheral arterial chemoreceptors whose function remains controversial, partly because information on their cellular physiology is lacking. In this study, we used ratiometric Ca2+ imaging to investigate for the first time chemosensitivity in short-term cultures of dissociated cells of juvenile rat ABs, located near the junction of the left vagus and recurrent laryngeal nerves. Among the surviving cell population were glomus or type I cell clusters, endogenous local neurons and glia-like cells. A variety of chemostimuli, including hypoxia, isohydric or acidic hypercapnia, and isocapnic acidosis, caused a rise in intracellular [Ca2+] in AB type I cells. The [Ca2+]i responses were indistinguishable from those in carotid body (CB) type I cells grown in parallel cultures from the same animals, and responses to acidic hypercapnia were prevented by the non-specific voltage-gated Ca2+ channel antagonist, 2mM Ni2+. Furthermore, we identified a subpopulation (∼40%) of glia-like cells in AB cultures that resembled CB type II cells based on their approximately equal sensitivity to ATP and UTP, consistent with the expression of purinergic P2Y2 receptors. Finally, we showed that some local neurons, known to be uniquely associated with these AB paraganglia in situ, generated robust [Ca2+]i responses to these chemostimuli. Thus, these AB type I cells and associated putative type II cells resemble those from the well-studied CB. Unlike the CB, however, they also associate with a special group of endogenous neurons which we propose may subserve a sensory function in local cardiovascular reflexes.
Authors:
Nikol A Piskuric; Colin A Nurse
Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2012-03-19
Journal Detail:
Title:  The Journal of physiology     Volume:  590     ISSN:  1469-7793     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-02     Completed Date:  2012-11-08     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2121-35     Citation Subset:  IM    
Affiliation:
Department of Biology, McMaster University, Life Sciences Building, Hamilton, Ontario, Canada. piskurn@mcmaster.ca
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MeSH Terms
Descriptor/Qualifier:
Acidosis / metabolism
Adenosine Triphosphate / metabolism
Animals
Aortic Bodies / drug effects,  metabolism*
Calcium / metabolism*
Calcium Channel Blockers / pharmacology
Calcium Signaling* / drug effects
Carotid Body / drug effects,  metabolism*
Cell Hypoxia
Cells, Cultured
Fluorescent Antibody Technique
Hydrogen-Ion Concentration
Hypercapnia / metabolism
Microscopy, Confocal
Microscopy, Fluorescence
Oxygen / metabolism*
Rats
Rats, Wistar
Receptors, Purinergic P2Y2 / metabolism
Sensory Receptor Cells / drug effects,  metabolism*
Time Factors
Uridine Triphosphate / metabolism
Chemical
Reg. No./Substance:
0/Calcium Channel Blockers; 0/Receptors, Purinergic P2Y2; 56-65-5/Adenosine Triphosphate; 63-39-8/Uridine Triphosphate; 7440-70-2/Calcium; 7782-44-7/Oxygen
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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