Document Detail

Effects of chartreusin on cell survival and cell cycle progression.
MedLine Citation:
PMID:  567096     Owner:  NLM     Status:  MEDLINE    
Chartreusin was lethal to both L1210 and P388 cells in culture with 90% of the cells being killed after a 24-hr exposure to 1.1 and 2.6 microgram/ml, respectively. The lethality of the drug increased in direct proportion to dose and exposure time. Both L1210 and Chinese hamster ovary cells in S phase were more sensitive to the lethality of the drug than were their corresponding non-S-phase cells. L1210 cells were partially synchronized by exposing an asynchronous culture to [methyl-3H]thymidine (20 Ci/mmol) and Colcemid for 3 hr. Synchronous culture of Chinese hamster ovary cells was established by planting mitotic cells. The progression of cells through the cell cycle was studied with flow microfluorometry both in the presence of the drug and after the drug had been washed off. In the presence of chartreusin the progression of mitotic cells into G1 was not affected. The movement of G1 cells into S was slower, and the movement of G2 cells into mitosis was blocked. When the drug was removed, the G2 to M block persisted for at least 4 hr but the progression of G1 cells to S was no longer inhibited.
B K Bhuyan; M I Robinson; K D Shugars; V H Bono; R L Dion
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  38     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1978 Sep 
Date Detail:
Created Date:  1978-10-27     Completed Date:  1978-10-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2734-9     Citation Subset:  IM    
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MeSH Terms
Antibiotics, Antineoplastic / pharmacology*
Cell Cycle / drug effects*
Cell Survival / drug effects*
Cells, Cultured
DNA, Neoplasm / metabolism
Demecolcine / pharmacology
Leukemia L1210 / drug therapy
Leukemia, Experimental / drug therapy*,  metabolism,  pathology
Thymidine / pharmacology
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 0/DNA, Neoplasm; 0/Glycosides; 477-30-5/Demecolcine; 50-89-5/Thymidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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