Document Detail


Effects on cell viability of three zebrafish testicular cell or tissue cryopreservation methods.
MedLine Citation:
PMID:  19448864     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this work, three cryopreservation procedures were tested in order to obtain efficiently viable testicular cells after cryopreservation. Testicular cells of Wild type zebrafish males were frozen using an equilibrium protocol and testicular tissue fragments were cryopreserved with equilibrium freezing and vitrification procedures. Results showed that vitrification was significantly more efficient than freezing in terms of final cell survival (cell freezing: 14.4%, tissue freezing: 77.4%-85.5%, tissue vitrification: 94%). It must be noted that, in live cells, the presence of pseudopodia was frequently observed, which indicated their spermatogonial nature. Based on these results, the authors suggest that vitrification, with the subsequent elimination of connective tissue after warming, offers the best combination to rescue live testicular cells as a genetic conservation procedure in zebrafish.
Authors:
C Bono-Mestre; J Cardona-Costa; F García-Ximénez
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cryo letters     Volume:  30     ISSN:  0143-2044     ISO Abbreviation:  Cryo Letters     Publication Date:    2009 Mar-Apr
Date Detail:
Created Date:  2009-05-18     Completed Date:  2009-07-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9891832     Medline TA:  Cryo Letters     Country:  England    
Other Details:
Languages:  eng     Pagination:  148-52     Citation Subset:  IM    
Affiliation:
Laboratory of Animal Reproduction and Biotechnology (LARB-UPV), Polytechnic University of Valencia, Valencia, Spain. bono-mestre@hotmail.es
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Survival / drug effects*
Cryopreservation / methods*
Cryoprotective Agents / pharmacology
Male
Testis / cytology*,  drug effects
Zebrafish / growth & development*
Chemical
Reg. No./Substance:
0/Cryoprotective Agents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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