Document Detail


Effects of cell-type-specific expression of a pan-caspase inhibitor on renal fibrogenesis.
MedLine Citation:
PMID:  25070875     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: The caspase family of enzymes is grouped into two major sub-families, namely apoptotic and inflammatory caspases, which play central roles in the induction of apoptosis, regulation of inflammation and immunity, and cellular differentiation.
METHODS: The role of caspase activation in tubular epithelium and interstitial cells of 3 lines of transgenic mice with obstructed nephropathy was examined: p35 mice bearing the pan-caspase inhibitor protein expressed by the p35 gene separated from the universal CAG promoter by a floxed STOP sequence were crossed with γGT.Cre and FSP1.Cre mice that express Cre recombinase in the cortical tubular epithelium and FSP1(+) interstitial cells, respectively. The γGT.Cre;p35, FSP1.Cre;p35 and p35 control mice were then challenged with unilateral ureter obstruction (UUO).
RESULTS: Proinflammatory parameters such as protein levels of active IL-1β subunit and mRNA levels of TNF-α and NOD-like receptor pyrin domain containing-3, and profibrogenic parameters such as interstitial matrix deposition and mRNA levels of fibronectin EIIIA isoform and α1 chain of procollagen type I in the kidneys were significantly increased at 7 days in the FSP1.Cre;p35- and p35-UUO mice, but not in the γGT.Cre;p35-UUO mice. These changes paralleled the numbers of apoptotic nuclei in tubules, but not in interstitial cells, and the protein levels of active caspase-3 subunit in the kidneys of FSP1.Cre;p35-, p35- and γGT.Cre;p35-UUO mice.
CONCLUSION: This study provides evidence of the critical role of caspase activation in the tubular epithelium, but not in FSP1(+) interstitial cells, in apoptosis and inflammasome induction, leading to proinflammatory and profibrogenic processes in fibrous kidneys with UUO.
Authors:
Tsutomu Inoue; Takeru Kusano; Kouji Tomori; Hidetomo Nakamoto; Hiromichi Suzuki; Hirokazu Okada
Related Documents :
25020525 - P106loss of myocardial nnos in human atrial fibrillation (af) shortens action potential...
24824215 - Genetic deletion of tnfrii gene enhances the alzheimer-like pathology in an app transge...
23849445 - Effect of immunodeficiency on mpv shedding and transmission.
23440245 - A dual role for hdac1: oncosuppressor in tumorigenesis, oncogene in tumor maintenance.
25278835 - In vivo overexpression of x-linked inhibitor of apoptosis protein protects against neom...
18004725 - Transplanted astrocytes internalize deposited beta-amyloid peptides in a transgenic mou...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-7-29
Journal Detail:
Title:  Clinical and experimental nephrology     Volume:  -     ISSN:  1437-7799     ISO Abbreviation:  Clin. Exp. Nephrol.     Publication Date:  2014 Jul 
Date Detail:
Created Date:  2014-7-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9709923     Medline TA:  Clin Exp Nephrol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  IgG sera levels against a subset of periodontopathogens and severity of disease in aggressive period...
Next Document:  Intestinal barrier dysfunction in HIV infection: pathophysiology, clinical implications and potentia...