Document Detail

Effects of cell proliferation on the uptake of transferrin-bound iron by human hepatoma cells.
MedLine Citation:
PMID:  14512884     Owner:  NLM     Status:  MEDLINE    
The effects of cellular proliferation on the uptake of transferrin-bound iron (Tf-Fe) and expression of transferrin receptor-1 (TfR1) and transferrin receptor-2 (TfR2) were investigated using a human hepatoma (HuH7) cell line stably transfected with TfR1 antisense RNA expression vector to suppress TfR1 expression. At transferrin (Tf) concentrations of 50 nmol/L and 5 micromol/L, when Tf-Fe uptake occurs by the TfR1- and TfR1-independent (NTfR1)-mediated process, respectively, the rate of Fe uptake by proliferating cells was approximately 250% that of stationary cells. The maximum rate of Fe uptake by the TfR1- and NTfR1-mediated process by proliferating cells was increased to 200% and 300% that of stationary cells, respectively. The maximum binding of Tf by both TfR1- and NTfR1-mediated processes by proliferating cells was increased significantly to 160% that of stationary cells. TfR1 and TfR2-alpha protein levels expressed by proliferating cells was observed to be approximately 300% and 200% greater than the stationary cells, respectively. During the proliferating growth phase, expression of TfR1 messenger RNA (mRNA) increased to 300% whereas TfR2-alpha mRNA decreased to 50% that of stationary cells. In conclusion, an increase in Tf-Fe uptake by TfR1-mediated pathway by proliferating cells was associated with increased TfR1 mRNA and protein expression. An increase in Tf-Fe uptake by NTfR1-mediated pathway was correlated with an increase in TfR2-alpha protein expression but not TfR2-alpha mRNA. In conclusion, TfR2-alpha protein is likely to have a role in the mediation of Tf-Fe uptake by the NTfR1 process by HuH7 hepatoma cell in proliferating and stationary stages of growth.
Adrian W M Lee; Phillip S Oates; Deborah Trinder
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Hepatology (Baltimore, Md.)     Volume:  38     ISSN:  0270-9139     ISO Abbreviation:  Hepatology     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-09-26     Completed Date:  2003-10-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8302946     Medline TA:  Hepatology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  967-77     Citation Subset:  IM    
Department of Physiology, School of Biomedical and Chemical Sciences, The University of Western Australia, Crawley, Western Australia, Australia.
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MeSH Terms
Carcinoma, Hepatocellular / metabolism*
Cell Division
Iron / metabolism*
Liver Neoplasms / metabolism*
RNA, Messenger / analysis
Receptors, Transferrin / genetics,  physiology
Transferrin / metabolism*
Tumor Cells, Cultured
Reg. No./Substance:
0/RNA, Messenger; 0/Receptors, Transferrin; 0/TFR2 protein, human; 11096-37-0/Transferrin; 7439-89-6/Iron

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