Document Detail

Effects of cations on antimicrobial activity of ostricacins-1 and 2 on E. coli O157:H7 and S. aureus 1056MRSA.
MedLine Citation:
PMID:  17554470     Owner:  NLM     Status:  MEDLINE    
Ostricacin-1 and ostricacin-2 (Osp-1 and Osp-2) were beta-defensins antimicrobial peptides that were purified from ostrich leukocytes using a cation-exchange column and a semi-prep RP-HPLC column. Both ostricacins were subjected to increased concentrations of monovalent cations (K(+) and Na(+)) and divalent cations (Ca(2+) and Mg(2+)) in order to investigate the effect of cations on the activity of these ostricacins on Gram-negative bacteria and Gram-positive bacteria. The radial diffusion assay method showed that both ostricacins were sensitive to the presence of cations. The divalent cations showed more antagonized effect on the activity against Gram-negative bacteria than the monovalent cations, as the ostricacins lost ability to inhibit bacterial growth at very low concentration (5 mM). When viewed in the context of other defensins activity, our data support a hypothesis that defensins' overall net positive charge determine the sensitivity to cations.
Haryadi Sugiarto; Pak-Lam Yu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-06
Journal Detail:
Title:  Current microbiology     Volume:  55     ISSN:  0343-8651     ISO Abbreviation:  Curr. Microbiol.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-06-18     Completed Date:  2007-10-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7808448     Medline TA:  Curr Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  36-41     Citation Subset:  IM    
Biotechnology Group, Institute of Technology and Engineering, Massey University, Private Bag 11-222, Palmerston North, 5301, New Zealand.
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MeSH Terms
Amino Acid Sequence
Anti-Bacterial Agents / antagonists & inhibitors*,  pharmacology
Cations / pharmacology*
Drug Antagonism
Escherichia coli O157 / drug effects*
Leukocytes / chemistry
Metals / pharmacology*
Microbial Sensitivity Tests
Molecular Sequence Data
Staphylococcus aureus / drug effects*
beta-Defensins / antagonists & inhibitors*,  chemistry,  pharmacology
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Cations; 0/Metals; 0/beta-Defensins

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