| Effects of cardiotrophin-1 on differentiation and maturation of rat bone marrow mesenchymal stem cells induced with 5-azacytidine in vitro. | |
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MedLine Citation:
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PMID: 19269704 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Cardiotrophin-1 (CT-1) is a cytokine involved in the growth and survival of cardiac cells that stimulates cardiomyogenesis in pluripotent murine embryonic stem (ES) cells. But it is not known whether CT-1 is responsible for the fate of differentiated bone marrow mesenchymal stem cells (BMMSCs). METHODS: We investigated the effects of CT-1 on differentiation and maturation of BMMSCs in vitro induced with 5-azacytidine. BMMSCs isolated from femur of rats were induced by CT-1 only, by 5-azacytidine with or without CT-1,and an untreated control group was also set. After 4 weeks of induced culturing, we observed the levels of alpha-cardiac actin and troponin-I by immunohistochemical staining, the ultrastructure of induce-cultured BMMSCs, and the expression of GATA4, Nkx2.5, beta-myosin heavy chain (beta-MHC) and alpha-cardiac actin mRNA by real time RT-PCR analysis. RESULTS: Differentiated BMMSCs treated by 5-azacytidine and CT-1 distinctly showed formations of myofilaments and myotube-like structures-morphological characteristics of myocyte like cells, and spontaneous contraction of a few cells was observed. The protein levels of alpha-cardiac actin and troponin-T were significantly higher than control. Furthermore, mRNA expression of GATA4, Nkx2.5, alpha-cardiac actin and beta-MHC was increased remarkably. CONCLUSIONS: This study suggests that induced culturing of BMMSCs in the presence of 5-azacytidine combined with CT-1 can enhance cardiomyocytic characteristics. CT-1 upregulates the expression of GATA4, Nkx2-5, alpha-cardiac actin and beta-MHC mRNA, and rapidly promotes the differentiation and maturation of cardiomyocyte-like cells differentiated from BMMSCs induced with 5-azacytidine. |
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Authors:
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Chen Xinyun; Zeng Zhi; Zhou Bin; Rao Li; Chen Yucheng; Yan Yafei; Zhang Tingjie; Li Shengfu |
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Publication Detail:
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Type: In Vitro; Journal Article Date: 2009-03-09 |
Journal Detail:
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Title: International journal of cardiology Volume: 143 ISSN: 1874-1754 ISO Abbreviation: Int. J. Cardiol. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-23 Completed Date: 2010-12-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8200291 Medline TA: Int J Cardiol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 171-7 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2009 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Department of Cardiology, West China Hospital, Sichuan University, Chengdu 610041, China. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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genetics Animals Azacitidine / pharmacology* Bone Marrow Cells / cytology, drug effects Cell Differentiation / drug effects Cell Survival / drug effects Cells, Cultured Cytokines / pharmacology* Enzyme Inhibitors / pharmacology GATA4 Transcription Factor / genetics Gene Expression / drug effects Homeodomain Proteins / genetics Male Mesenchymal Stem Cells / cytology*, drug effects* Myosin Heavy Chains / genetics Rats Rats, Sprague-Dawley Transcription Factors / genetics |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Bmyo protein, rat; 0/Cytokines; 0/Enzyme Inhibitors; 0/GATA4 Transcription Factor; 0/Gata4 protein, rat; 0/Homeodomain Proteins; 0/Myosin Heavy Chains; 0/Nkx2.5 protein, rat; 0/Transcription Factors; 0/cardiotrophin 1; 320-67-2/Azacitidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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