Document Detail

Effects of carboxymethylcellulose and hydroxypropylmethylcellulose on the differentiation and activity of osteoclasts and osteoblasts.
MedLine Citation:
PMID:  20665677     Owner:  NLM     Status:  MEDLINE    
Carboxymethylcellulose (CMC) and hydroxypropylmethylcellulose (HPMC) serve as carriers for growth factors and bone substitutes. Although both carriers are placed into the defect sites, their impacts on bone regeneration are unclear. Herein, we examined whether CMC and HPMC affect the differentiation of bone marrow progenitors into osteoclasts and osteoblasts. We therefore induced osteoclastogenesis and osteoblastogenesis in murine bone marrow progenitors in the presence of CMC and HPMC, respectively. Measures of osteoclastogesis were based on the number and activity of tartrate-resistant acid-phosphatase-positive (TRAP(+)) multinucleated cells and expression of marker genes. Osteoblastogenesis was determined by the number and activity of alkaline-phosphatase-positive (AP(+)) colonies and relevant marker genes. Viability was assessed by colorimetric measurement of formazan formation. We report that CMC at 1% caused a significant reduction in the number and activity of TRAP(+) multinucleated cells. Changes in viability were not responsible for the observed effects. HPMC showed no remarkable impact on osteoclastogenesis; however, the concentration was limited to 0.5% because of the high viscosity. The ability of bone marrow progenitors to form AP(+) colonies was not affected by either of the two carriers. Together, these results suggest that CMC and possibly also HPMC can decrease osteoclastogenesis while osteoblastogenesis remains unchanged in vitro. These observations raise the possibility that these carriers might affect the cellular process of bone regeneration.
Hermann Agis; Benedict Beirer; Georg Watzek; Reinhard Gruber
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  95     ISSN:  1552-4965     ISO Abbreviation:  J Biomed Mater Res A     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-09-22     Completed Date:  2011-01-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  504-9     Citation Subset:  IM    
Department of Oral Surgery, Medical University of Vienna, Austria.
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MeSH Terms
Biocompatible Materials / chemistry,  metabolism
Biological Markers / metabolism
Bone Marrow Cells / cytology,  physiology
Bone Regeneration / drug effects*
Carboxymethylcellulose Sodium / pharmacology*
Cell Differentiation / drug effects*
Cell Survival
Cells, Cultured
Drug Carriers / chemistry,  pharmacology
Materials Testing
Methylcellulose / analogs & derivatives*,  pharmacology
Ophthalmic Solutions / pharmacology
Osteoblasts / cytology,  drug effects*,  physiology
Osteoclasts / cytology,  drug effects*,  physiology
Stem Cells / cytology,  physiology
Reg. No./Substance:
0/Biocompatible Materials; 0/Biological Markers; 0/Drug Carriers; 0/Ophthalmic Solutions; 8063-82-9/hypromellose; 9004-32-4/Carboxymethylcellulose Sodium; 9004-67-5/Methylcellulose

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