Document Detail


Effects of carboxymethylcellulose and carboxypolymethylene on morphology of Aspergillus fumigatus NRRL 2346 and fumagillin production.
MedLine Citation:
PMID:  12432459     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Aspergillus fumigatus NRRL 2346 is the producer of fumagillin, an antitumor antibiotic that inhibits angiogenesis. This strain is very difficult to grow reproducibly in shake flasks owing to an extreme form of pellet growth and extensive wall growth. The effects of carboxymethylcellulose (CMC) and carboxypolymethylene (Carbopol) on growth and fumagillin production by A. fumigatus were investigated. By adding the polymers to the fermentation medium, the growth form of the mold was changed from a single large glob to small reproducible pellets, and wall growth was diminished to a minimum. Carbopol, at a lower concentration, was more effective than CMC in improving both morphology and production. Small pellets were produced which favored fumagillin biosynthesis. 1.5% (wt/vol) CMC and 0.3% (wt/vol) Carbopol were found to be the optimum concentrations; higher levels increased viscosity to an unacceptable level.
Authors:
Wen Yang; Erika A Hartwieg; Aiqi Fang; Arnold L Demain
Related Documents :
1138829 - Effect of frusemide on fetal urine production.
11228509 - Vasoactive mediator release by fetal endothelial cells in intrauterine growth restricti...
22871339 - Expressions of vitamin d metabolic components vdbp, cyp2r1, cyp27b1, cyp24a1, and vdr i...
11536139 - Microbial growth by a net heat up-take: a calorimetric and thermodynamic study on aceto...
9219449 - Intrauterine growth pattern by the tendency to repeat small-for-gestational-age births ...
20004469 - Review: sex and the human placenta: mediating differential strategies of fetal growth a...
8571849 - Fetal lower urinary tract physiology: in vivo studies.
16964809 - Substance abuse in pregnancy: opioid substitution in a northern ireland maternity unit.
22388229 - Effects of maternal smoking during pregnancy on offspring blood pressure in late adoles...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current microbiology     Volume:  46     ISSN:  0343-8651     ISO Abbreviation:  Curr. Microbiol.     Publication Date:  2003 Jan 
Date Detail:
Created Date:  2002-11-14     Completed Date:  2003-04-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7808448     Medline TA:  Curr Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  24-7     Citation Subset:  IM; S    
Affiliation:
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / biosynthesis*
Aspergillus fumigatus / cytology,  drug effects*,  metabolism
Carboxymethylcellulose / pharmacology*
Cyclohexanes
Dose-Response Relationship, Drug
Fatty Acids, Unsaturated / biosynthesis*
Hyphae / physiology
Polyvinyls / pharmacology*
Sesquiterpenes
Spores, Fungal / physiology
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Cyclohexanes; 0/Fatty Acids, Unsaturated; 0/Polyvinyls; 0/Sesquiterpenes; 23110-15-8/fumagillin; 9004-32-4/Carboxymethylcellulose; 9007-20-9/carboxypolymethylene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Factors affecting the antibacterial activity of the ruminal bacterium, Streptococcus bovis HC5.
Next Document:  Biofilm formation and biocide susceptibility testing of Mycobacterium fortuitum and Mycobacterium ma...