Document Detail

Effects of carboxymethylcellulose and carboxypolymethylene on morphology of Aspergillus fumigatus NRRL 2346 and fumagillin production.
MedLine Citation:
PMID:  12432459     Owner:  NLM     Status:  MEDLINE    
Aspergillus fumigatus NRRL 2346 is the producer of fumagillin, an antitumor antibiotic that inhibits angiogenesis. This strain is very difficult to grow reproducibly in shake flasks owing to an extreme form of pellet growth and extensive wall growth. The effects of carboxymethylcellulose (CMC) and carboxypolymethylene (Carbopol) on growth and fumagillin production by A. fumigatus were investigated. By adding the polymers to the fermentation medium, the growth form of the mold was changed from a single large glob to small reproducible pellets, and wall growth was diminished to a minimum. Carbopol, at a lower concentration, was more effective than CMC in improving both morphology and production. Small pellets were produced which favored fumagillin biosynthesis. 1.5% (wt/vol) CMC and 0.3% (wt/vol) Carbopol were found to be the optimum concentrations; higher levels increased viscosity to an unacceptable level.
Wen Yang; Erika A Hartwieg; Aiqi Fang; Arnold L Demain
Related Documents :
3321859 - Perinatal outcome in growth retarded pregnancies dated by ultrasound.
11844169 - A prospective longitudinal study of growth velocity in twin pregnancy.
1806139 - Parental epilepsy, anticonvulsant drugs, and reproductive outcome: epidemiologic and ex...
19031279 - Serum fetuin-a/alpha2-hs-glycoprotein in human pregnancies with normal and restricted f...
9989859 - Fetal growth and long-term consequences in animal models of growth retardation.
17694329 - Effects of experimental duration and density of japanese dace tribolodon hakonensis on ...
11853029 - Endothelin-mediated preeclampsia at high altitude.
6436069 - The effect of different methods of ovarian stimulation for human in vitro fertilization...
14770389 - Relationship between monitoring parameters and perinatal outcome in severe, early intra...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current microbiology     Volume:  46     ISSN:  0343-8651     ISO Abbreviation:  Curr. Microbiol.     Publication Date:  2003 Jan 
Date Detail:
Created Date:  2002-11-14     Completed Date:  2003-04-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7808448     Medline TA:  Curr Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  24-7     Citation Subset:  IM; S    
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Anti-Bacterial Agents / biosynthesis*
Aspergillus fumigatus / cytology,  drug effects*,  metabolism
Carboxymethylcellulose / pharmacology*
Dose-Response Relationship, Drug
Fatty Acids, Unsaturated / biosynthesis*
Hyphae / physiology
Polyvinyls / pharmacology*
Spores, Fungal / physiology
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Cyclohexanes; 0/Fatty Acids, Unsaturated; 0/Polyvinyls; 0/Sesquiterpenes; 23110-15-8/fumagillin; 9004-32-4/Carboxymethylcellulose; 9007-20-9/carboxypolymethylene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Factors affecting the antibacterial activity of the ruminal bacterium, Streptococcus bovis HC5.
Next Document:  Biofilm formation and biocide susceptibility testing of Mycobacterium fortuitum and Mycobacterium ma...