Document Detail

Effects of carboxymethylcellulose and carboxypolymethylene on morphology of Aspergillus fumigatus NRRL 2346 and fumagillin production.
MedLine Citation:
PMID:  12432459     Owner:  NLM     Status:  MEDLINE    
Aspergillus fumigatus NRRL 2346 is the producer of fumagillin, an antitumor antibiotic that inhibits angiogenesis. This strain is very difficult to grow reproducibly in shake flasks owing to an extreme form of pellet growth and extensive wall growth. The effects of carboxymethylcellulose (CMC) and carboxypolymethylene (Carbopol) on growth and fumagillin production by A. fumigatus were investigated. By adding the polymers to the fermentation medium, the growth form of the mold was changed from a single large glob to small reproducible pellets, and wall growth was diminished to a minimum. Carbopol, at a lower concentration, was more effective than CMC in improving both morphology and production. Small pellets were produced which favored fumagillin biosynthesis. 1.5% (wt/vol) CMC and 0.3% (wt/vol) Carbopol were found to be the optimum concentrations; higher levels increased viscosity to an unacceptable level.
Wen Yang; Erika A Hartwieg; Aiqi Fang; Arnold L Demain
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current microbiology     Volume:  46     ISSN:  0343-8651     ISO Abbreviation:  Curr. Microbiol.     Publication Date:  2003 Jan 
Date Detail:
Created Date:  2002-11-14     Completed Date:  2003-04-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7808448     Medline TA:  Curr Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  24-7     Citation Subset:  IM; S    
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
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MeSH Terms
Anti-Bacterial Agents / biosynthesis*
Aspergillus fumigatus / cytology,  drug effects*,  metabolism
Carboxymethylcellulose / pharmacology*
Dose-Response Relationship, Drug
Fatty Acids, Unsaturated / biosynthesis*
Hyphae / physiology
Polyvinyls / pharmacology*
Spores, Fungal / physiology
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Cyclohexanes; 0/Fatty Acids, Unsaturated; 0/Polyvinyls; 0/Sesquiterpenes; 23110-15-8/fumagillin; 9004-32-4/Carboxymethylcellulose; 9007-20-9/carboxypolymethylene

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