Document Detail


Effects of candidate vaginally-applied microbicide compounds on innate immune cells.
MedLine Citation:
PMID:  16040128     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ideally, a vaginally-applied microbicide would be effective against a broad range of pathogens but would have minimal effects on the female genital tract. The aim of this study was to determine if representative candidate detergent-type and sulfated/sulfonated polymer-type microbicides altered the composition or function of innate immune cells normally found in the vaginal mucosa. The effect of microbicide on the composition of vaginal leukocytes was tested using a flow cytometric approach. Application of the detergent cholic acid, but not the sulfated polysaccharide lambda carrageenan, resulted in a significant increase in macrophages at the vaginal epithelial surface compared to control treatment (19.3% macrophages compared to 2.8%; p<0.0004). Phagocytosis of fluorochrome-labeled bacteria by macrophages was inhibited greater than 50% in the presence of 1.0mg/ml of the sulfonated polymer PRO 2000 but was not inhibited by the same concentration of lambda carrageenan. PRO 2000-pulsed macrophages regained phagocytic function after being washed free of the compound. Culture of macrophages with PRO 2000 also resulted in diminished detection of the surface proteins CD11b and CD18. After treated cells were washed free of PRO 2000, these proteins were detected at levels similar to control treated cells. In conclusion, application of a detergent-type microbicide, but not a sulfated polymer, resulted in the infiltration of inflammatory cells at the vaginal epithelial surface. Phagocytic function of macrophages was lost in the presence of 1mg/ml PRO 2000 which may have reflected masking of important cell surface proteins by the microbicide; however, there was no evidence of permanent loss of function upon removal of the compound.
Authors:
Gregg N Milligan; Christal G Young; Michael G Meador; Chin-Fun Chu; Lawrence R Stanberry
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of reproductive immunology     Volume:  66     ISSN:  0165-0378     ISO Abbreviation:  J. Reprod. Immunol.     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-08-01     Completed Date:  2005-12-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8001906     Medline TA:  J Reprod Immunol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  103-16     Citation Subset:  IM    
Affiliation:
Sealy Center for Vaccine Development, 301 University Boulevard, Galveston, TX 77555-0436, USA. gnmillig@utmb.edu
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MeSH Terms
Descriptor/Qualifier:
Administration, Intravaginal
Animals
Anti-Infective Agents, Local / administration & dosage,  toxicity*
Carrageenan / administration & dosage,  toxicity
Cholic Acid / administration & dosage,  toxicity
Detergents / administration & dosage,  toxicity
Female
Flow Cytometry
Immunity, Innate / drug effects*
Macrophages / drug effects*,  metabolism,  microbiology
Mice
Mucous Membrane / cytology,  microbiology
Naphthalenesulfonates / administration & dosage,  toxicity
Phagocytosis / drug effects
Polymers / administration & dosage,  toxicity
Polystyrenes / administration & dosage,  toxicity
Vagina / cytology,  immunology*,  microbiology
Grant Support
ID/Acronym/Agency:
AI-054444/AI/NIAID NIH HHS; AI-37940/AI/NIAID NIH HHS; AI-42815/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Anti-Infective Agents, Local; 0/Detergents; 0/Naphthalenesulfonates; 0/PRO 2000; 0/Polymers; 0/Polystyrenes; 28210-41-5/polystyrene sulfonic acid; 81-25-4/Cholic Acid; 9000-07-1/Carrageenan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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