| Effects of cAMP on intercellular coupling and osteoblast differentiation. | |
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MedLine Citation:
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PMID: 11302734 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bone-forming cells are organized in a multicellular network interconnected by gap junctions. Direct intercellular communication via gap junctions is an important component of bone homeostasis, coordinating cellular responses to external signals and promoting osteoblast differentiation. The cAMP pathway, a major intercellular signal transduction mechanism, regulates osteoblastic function and metabolism. We investigated the effects of this second messenger on junctional communication and on the expression of differentiation markers in human HOBIT osteoblastic cells. Increased levels of cAMP induce posttranslational modifications (i.e., phosphorylations) of connexin43 and enhancement of gap junction assembly, resulting in an increased junctional permeance to Lucifer yellow and to a positive modulation of intercellular Ca(2+) waves. Increased intercellular communication, however, was accompanied by a parallel decrease of alkaline phosphatase activity and by an increase of osteocalcin expression. cAMP-dependent stimulation of cell-to-cell coupling induces a complex modulation of bone differentiation markers. |
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Authors:
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M Romanello; L Moro; D Pirulli; S Crovella; P D'Andrea |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 282 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2001 Apr |
Date Detail:
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Created Date: 2001-04-16 Completed Date: 2001-05-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 1138-44 Citation Subset: IM |
Copyright Information:
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Copyright 2001 Academic Press. |
Affiliation:
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Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Università di Trieste, via Licio Giorgieri 1, Trieste, I-34127, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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antagonists & inhibitors,
metabolism Antigens, Differentiation / metabolism Calcium Signaling / drug effects Cell Communication / drug effects, physiology* Cell Differentiation / drug effects, physiology* Cell Line Cell Membrane Permeability / drug effects Connexin 43 / metabolism Cyclic AMP / metabolism*, pharmacology Forskolin / pharmacology Gap Junctions / drug effects Humans Osteoblasts / cytology, drug effects, metabolism* Osteocalcin / genetics, metabolism Phosphodiesterase Inhibitors / pharmacology Phosphorylation / drug effects RNA, Messenger / metabolism Signal Transduction / drug effects, physiology |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Differentiation; 0/Connexin 43; 0/Phosphodiesterase Inhibitors; 0/RNA, Messenger; 104982-03-8/Osteocalcin; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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