Document Detail


Effects of bone sialoprotein on pancreatic cancer cell growth, invasion and metastasis.
MedLine Citation:
PMID:  16488077     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bone sialoprotein (BSP) is an acidic glycoprotein that plays an important role in cancer cell growth, migration and invasion. The expression, localization and possible function of BSP in chronic pancreatitis (CP) and pancreatic ductal adenocarcinoma (PDAC) were analyzed by QRT-PCR, laser capture microdissection, DNA microarray analysis, immunoblotting, radioimmunoassays and immunohistochemistry as well as cell growth, invasion, scattering, and adhesion assays. BSP mRNA was detected in 40.7% of normal, in 80% of CP and in 86.4% of PDAC samples. The median BSP mRNA levels were 6.1 and 0.9copies/microl cDNA in PDAC and CP tissues, respectively, and zero copies/microl cDNA in normal pancreatic tissues. BSP was weakly present in the cytoplasm of islet cells and ductal cells in 20% of normal pancreatic tissues. BSP was localized in the tubular complexes of both CP and PDAC, as well as in pancreatic cancer cells. Five out of 8 pancreatic cancer cell lines expressed BSP mRNA. Recombinant BSP (rBSP) inhibited Capan-1 and SU8686 pancreatic cancer cell growth, with a maximal effect of -46.4+/-12.0% in Capan-1 cells and -45.7+/-14.5% in SU8686 cells. rBSP decreased the invasion of SU8686 cells by -59.1+/-11.2% and of Capan-1 cells by -13.3+/-3.8% (P<0.05), whereas it did not affect scattering or adhesion of both cell lines. In conclusion, endogenous BSP expression levels in pancreatic cancer cells and low to absent BSP expression in the surrounding stromal tissue elements may indirectly act to enhance the proliferation and invasion of pancreatic cancer cells.
Authors:
Hany Kayed; Jörg Kleeff; Shereen Keleg; Klaus Felix; Thomas Giese; Martin R Berger; Markus W Büchler; Helmut Friess
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-02-20
Journal Detail:
Title:  Cancer letters     Volume:  245     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2006-12-25     Completed Date:  2007-04-05     Revised Date:  2010-02-04    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  171-83     Citation Subset:  IM    
Affiliation:
Department of General Surgery, University of Heidelberg, Im Neuenheimer Feld 110, 69120 Heidelberg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Carcinoma, Pancreatic Ductal / genetics,  metabolism,  pathology*
Cell Adhesion
Cell Line, Tumor
Cell Movement* / drug effects
Cell Proliferation* / drug effects
Cell Survival / drug effects
DNA Methylation
Dose-Response Relationship, Drug
Gene Expression Regulation, Neoplastic
Humans
Immunoblotting
Immunohistochemistry
Neoplasm Invasiveness
Neoplasm Metastasis
Pancreatic Neoplasms / genetics,  metabolism,  pathology*
Promoter Regions, Genetic
RNA, Messenger / genetics,  metabolism
Radioimmunoassay
Recombinant Proteins / pharmacology
Reverse Transcriptase Polymerase Chain Reaction / methods
Sialoglycoproteins / blood,  genetics*,  metabolism
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/Recombinant Proteins; 0/Sialoglycoproteins; 0/integrin-binding sialoprotein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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