| Effects of bone sialoprotein on pancreatic cancer cell growth, invasion and metastasis. | |
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MedLine Citation:
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PMID: 16488077 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bone sialoprotein (BSP) is an acidic glycoprotein that plays an important role in cancer cell growth, migration and invasion. The expression, localization and possible function of BSP in chronic pancreatitis (CP) and pancreatic ductal adenocarcinoma (PDAC) were analyzed by QRT-PCR, laser capture microdissection, DNA microarray analysis, immunoblotting, radioimmunoassays and immunohistochemistry as well as cell growth, invasion, scattering, and adhesion assays. BSP mRNA was detected in 40.7% of normal, in 80% of CP and in 86.4% of PDAC samples. The median BSP mRNA levels were 6.1 and 0.9copies/microl cDNA in PDAC and CP tissues, respectively, and zero copies/microl cDNA in normal pancreatic tissues. BSP was weakly present in the cytoplasm of islet cells and ductal cells in 20% of normal pancreatic tissues. BSP was localized in the tubular complexes of both CP and PDAC, as well as in pancreatic cancer cells. Five out of 8 pancreatic cancer cell lines expressed BSP mRNA. Recombinant BSP (rBSP) inhibited Capan-1 and SU8686 pancreatic cancer cell growth, with a maximal effect of -46.4+/-12.0% in Capan-1 cells and -45.7+/-14.5% in SU8686 cells. rBSP decreased the invasion of SU8686 cells by -59.1+/-11.2% and of Capan-1 cells by -13.3+/-3.8% (P<0.05), whereas it did not affect scattering or adhesion of both cell lines. In conclusion, endogenous BSP expression levels in pancreatic cancer cells and low to absent BSP expression in the surrounding stromal tissue elements may indirectly act to enhance the proliferation and invasion of pancreatic cancer cells. |
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Authors:
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Hany Kayed; Jörg Kleeff; Shereen Keleg; Klaus Felix; Thomas Giese; Martin R Berger; Markus W Büchler; Helmut Friess |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-02-20 |
Journal Detail:
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Title: Cancer letters Volume: 245 ISSN: 0304-3835 ISO Abbreviation: Cancer Lett. Publication Date: 2007 Jan |
Date Detail:
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Created Date: 2006-12-25 Completed Date: 2007-04-05 Revised Date: 2010-02-04 |
Medline Journal Info:
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Nlm Unique ID: 7600053 Medline TA: Cancer Lett Country: Ireland |
Other Details:
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Languages: eng Pagination: 171-83 Citation Subset: IM |
Affiliation:
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Department of General Surgery, University of Heidelberg, Im Neuenheimer Feld 110, 69120 Heidelberg, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carcinoma, Pancreatic Ductal
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genetics,
metabolism,
pathology* Cell Adhesion Cell Line, Tumor Cell Movement* / drug effects Cell Proliferation* / drug effects Cell Survival / drug effects DNA Methylation Dose-Response Relationship, Drug Gene Expression Regulation, Neoplastic Humans Immunoblotting Immunohistochemistry Neoplasm Invasiveness Neoplasm Metastasis Pancreatic Neoplasms / genetics, metabolism, pathology* Promoter Regions, Genetic RNA, Messenger / genetics, metabolism Radioimmunoassay Recombinant Proteins / pharmacology Reverse Transcriptase Polymerase Chain Reaction / methods Sialoglycoproteins / blood, genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/Recombinant Proteins; 0/Sialoglycoproteins; 0/integrin-binding sialoprotein |
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